应用溴甲酚紫建立贵州省辣椒炭疽病菌快速选药技术研究  

Establishment of Technology of Rapid Fungicides Selection against Colletotrichum gloeosporioides in Guizhou Province Using Bromocresol Purple

作　　者：刘阿丽 吴小毛[1,2,3] 李荣玉 韩磊[1,2] 刘旭东 罗跃 LIU Ali;WU Xiaomao;LI Rongyu;HAN Lei;LIU Xudong;LUO Yue(Institute of Crop Protection,Guizhou University,Guiyang 550025,China;College of Agriculture,Guizhou University,Guiyang 550025,China;Key Laboratory for Agricultural Pest Management in Mountainous Region of Guizhou Province,Guiyang 550025,China)

机构地区：[1]贵州大学作物保护研究所,贵州贵阳550025 [2]贵州大学农学院,贵州贵阳550025 [3]贵州省山地农业病虫害重点实验室,贵州贵阳550025

出　　处：《河南农业科学》2021年第2期81-88,共8页Journal of Henan Agricultural Sciences

基　　金：贵州省成果转化项目(黔科合成果[2020]1Z002-3号)。

摘　　要：为了建立辣椒炭疽病快速选药技术,对贵州省4个辣椒主产区100株辣椒炭疽病菌菌株进行敏感性测定,建立了贵州省辣椒炭疽病菌对甲基硫菌灵和咪鲜胺的敏感基线,并以敏感基线为基准,应用溴甲酚紫为指示剂,研制出辣椒炭疽病菌快速选药试剂盒。结果表明,贵州省辣椒炭疽病菌对甲基硫菌灵和咪鲜胺的敏感基线分别为(0.7641±0.1508)μg/mL和(0.0710±0.0133)μg/mL。尖孢炭疽菌在0.45μg/mL甲基硫菌灵的试剂盒上颜色显示为橙红色表明比较敏感,在1.80μg/mL甲基硫菌灵的试剂盒上显示黄色则表明已经产生抗性,在0.088μg/mL咪鲜胺的试剂盒上显示橙红色表明比较敏感,在0.350μg/mL咪鲜胺的试剂盒上显示黄色则表明已经产生抗性。In order to establish the technology of rapid fungicides selection against Colletotrichum gloeosporioides,100 isolates of C.gloeosporioides were isolated and collected from Guizhou.The sensitivity baslines of C.gloeosporioides to thiophanate methyl and prochloraz were determined by mycelial growth rate method.Based on sensitivity basline of C.gloeosporioides to thiophanate methyl and prochloraz,the rapid fungicides selection kit against C.gloeosporioides was established using bromocresol purple.The results showed that the EC50 mean values of(0.7641±0.1508)μg/mL and(0.0710±0.0133)μg/mL were sensitivity baslines of C.gloeosporioides to thiophanate methyl,prochloraz in Guizhou region,respectively.When the strain of C.gloeosporioides was placed in selection kit of thiophanate methyl of 0.45μg/mL,the color of the kit was orange red,indicating that the strain of C.gloeosporioides to thiophanate methyl was sensitive.But when the strain of C.gloeosporioides was placed in selection kit of thiophanate methyl of 1.80μg/mL,the color of the kit was yellow,indicating that the strain was resistant.When the strain of C.gloeosporioides was placed in selection kit of prochloraz of 0.088μg/mL,the color of the kit was orange red,indicating that the strain of C.gloeosporioides to prochloraz was sensitive.But when the strain of C.gloeosporioides was placed in selection kit of prochloraz of 0.350μg/mL,the color of the kit was yellow,indicating that the strain was resistant.

关 键 词：辣椒炭疽病菌 甲基硫菌灵 咪鲜胺 敏感基线 溴甲酚紫 选药试剂盒 

分 类 号：S482.2[农业科学—农药学]