高盐对Dahl盐敏感大鼠肾脏Mcoln3表达的影响  

Overexpression of Mcoln3 in the kidney of Dahl salt sensitive rats during high salt diet

作　　者：朱纯锋 党斌涛 张先芹 刘琳 齐婷 张维[3] 唐治国[3] 马美娟[3] 刘富强[3] ZHU Chunfeng;DANG Bintao;ZHANG Xianqin;LIU Lin;QI Ting;ZHANG Wei;TANG Zhiguo;MA Meijuan;LIU Fuqiang(Department of Cardiology,Zhen’an County Hospital,Shangluo,Shangluo 711500,China)

机构地区：[1]陕西省商洛市镇安县医院心内科,711500 [2]陕西省西安市西安航天总医院急诊科 [3]陕西省人民医院心内一科

出　　处：《心肺血管病杂志》2021年第1期93-96,111,共5页Journal of Cardiovascular and Pulmonary Diseases

基　　金：国家自然基金青年科学基金项目(81200512);陕西省卫生计生科研基金项目(2016D034);中国博士后科学基金特别资助项目(2017T10078)。

摘　　要：目的:研究高盐饮食对Dahl盐敏感大鼠肾脏Mcoln3表达的影响。方法:将12只Dahl盐敏感大鼠和12只SS-13BN大鼠分别随机分为正常盐组、高盐组干预3周,采用免疫组化检测肾脏Mcoln3表达位置和表达差异,使用Real-time PCR和Western Blotting方法检测肾脏Mcoln3基因的mRNA和蛋白表达差异。结果:免疫组化显示Mcoln3蛋白在肾脏远端肾小管丰富表达,在高盐负荷后Dahl盐敏感大鼠肾小管Mcoln3蛋白表达明显较正常盐组增加,而13BN-SS大鼠组Mcoln3蛋白变化不明显;同样发现Dahl盐敏感大鼠肾脏Mcoln3的mRNA和蛋白表达在高盐负荷下较13BN-SS大鼠组显著增加,差异具有统计学意义(P<0.05)。结论:我们首次发现肾脏Mcoln3在Dahl盐敏感大鼠在盐负荷后表达异常,可能参与盐敏感性高血压的形成。Objective:To study the effect of high-salt diet on the expression of Mcoln3 in the kidney of Dahl salt-sensitive rats.Methods:Twelve Dahl salt-sensitive rats and SS-13 BN rats were randomly divided into normal salt group and high-salt group for 3 weeks of intervention.Immunohistochemistry was used to detect the expression position and difference of renal Mcoln3,using Real-time PCR and Western Blotting method were used to detect differences in the mRNA and protein expression of the kidney Mcoln3 gene.Results:Immunohistochemistry showed that Mcln3 protein was abundantly expressed in the distal renal tubules of the kidney.After high salt loading,the Mcoln3 protein expression in the renal tubules of Dahl salt-sensitive rats was significantly increased compared with the normal salt group,while the Mcoln3 protein in the 13 BN-SS rat group did not change;it was also found that the mRNA and protein expression of Mcoln3 in the kidney of Dahl salt-sensitive rats increased significantly compared with the 13 BN-SS rat group under high salt load(P<0.05).Conclusions:We found for the first time that renal Mcoln3 is abnormally expressed in Dahl salt-sensitive rats after salt loading,which may be involved in the formation of salt-sensitive hypertension.

关 键 词：盐敏感性高血压 Mcoln3 肾脏 钠钾代谢 

分 类 号：R54[医药卫生—心血管疾病]