兔背部皮下注射法建立增生性瘢痕动物模型  

Establishment of a novel hypertrophic scar animal model by subcutaneous injection on the back of rabbits

作　　者：姚庆 杨琼 李毅 彭干 郭梁影 蒋邦红 YAO Qing;YANG Qiong;LI Yi;PENG Gan;GUO Liang-ying;JIANG Bang-hong(School of Stomatology,Bengbu Medical College,Bengbu Anhui 233030,China)

机构地区：[1]蚌埠医学院口腔医学院,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2021年第2期270-272,共3页Journal of Bengbu Medical College

基　　金：安徽省高校省级大学生创新训练项目(201710367053);蚌埠医学院自然科学基金面上项目(BYKY1668);蚌埠医学院自然科学基金面上项目(BYKY1654)。

摘　　要：目的:探索无水乙醇皮下注射后对皮肤外形及形态的影响,建立简便易重复的瘢痕动物模型。方法:选取体质量均为1.5 kg的新西兰大白兔27只,雌雄不限,随机分为甲、乙、丙3组。将浓度为99.5%的乙醇按照1、2、3 mL/kg 3种剂量分别注入甲、乙、丙3组兔子背部皮肤真皮层,观察注射后7、30、90 d注射位点的变化,得出最佳造模方法。结果:乙组兔子30 d后肉眼可见类似人增生性瘢痕样组织形成,HE染色镜下可观察到表皮层增厚明显,真皮层中胶原纤维排列紊乱,Ⅰ型胶原纤维免疫组织化学染色,可见组织内Ⅰ型胶原蛋白染成棕黄色,细胞质内可见棕黄色颗粒,Ⅰ型胶原纤维数量明显增多。结论:2 mL/kg无水乙醇兔子背部皮下注射,经自然愈合后形成的增生性瘢痕与人体增生性瘢痕相似,可作为研究增生性瘢痕的发生机制及评估其治疗方法的动物模型之一。Objective:To explore the effects of anhydrous alcohol by subcutaneous injection on the appearance and morphology of skin,and establish a simple and repeatable animal model of scar.Methods:Twenty-seven New Zealand white rabbits,weighing 1.5 kg,were randomly divided into the A group,B group and C group.The 99.5%alcohol was injected into the dorsal dermis of rabbits in A,B and C groups at the doses of 1,2 and 3 mL/kg,respectively.The changes of injection sites at 7,30 and 90 d were observed according to the POSAS scoring standard and microscopic appearance for finding out the best dose.Results:The tissue similar to human hypertrophic scar in B group was found at 30 d of injection.The HE staining showed the epidermal layer significantly thickened,and the collagen fiber arrangement disorderedin the dermis.The results of typeⅠcollagen fiber IHC staining showed that the typeⅠcollagen was stained for brown,and the brown granules were found in the cytoplasm,and the number of typeⅠcollagen fibers increased significantly under the microscope.Conclusions:The hypertrophic scar formed by 2 mL/kg anhydrous alcoholic by subcutaneous injection is similar to the human hypertrophic scar,and can be used as one of the animal models in studying the mechanism of hypertrophic scar and evaluating the treatment.

关 键 词：瘢痕 动物模型 无水乙醇 兔 

分 类 号：R619.6[医药卫生—外科学]