木醋液对辣椒种子萌发及其抑菌作用的影响研究  

Study of wood vinegar effect on seeds germination of chili pepper and its antibacterial functions

作　　者：杜佳燕 武菊平 王婧瑶 耿丽平 李博文 刘文菊 DU Jiayan;WU Juping;WANG Jingyao;GENG Liping;LI Bowen;LIU Wenju(College of Recourses and Environmental Sciences,Hebei Agricultural University/Key Laboratory of Ecological Environment of Farmland in Hebei Province,Baoding 071000,China)

机构地区：[1]河北农业大学资源与环境科学学院/河北省农田生态环境重点实验室,河北保定071000

出　　处：《河北农业大学学报》2021年第1期91-96,共6页Journal of Hebei Agricultural University

基　　金：国家自然科学基金项目(31870490);河北省应用基础研究计划重点基础研究资助项目(17962902D);国家科技支撑计划项目(2015BAD23B01).

摘　　要：木醋液是活性炭生产的副产物。为实现废弃物资源再利用,本研究采用辣椒种子发芽试验和微生物平板培养试验,探究了木醋液对辣椒种子萌发、幼苗生长及对有益菌/病原菌的抑菌作用。结果表明:木醋液稀释100、250、500倍后浸种,与对照相比提高了辣椒种子发芽率、发芽指数、相对幼苗高度和根耐性指数,增幅分别为4.72%~10.38%、2.44%~13.06%、12.60%~39.45%和48.50%~132.34%,其中木醋液稀释250倍对辣椒种子萌发和幼苗生长促进效应最佳。此外,木醋液稀释250倍也促进了有益菌的生长,枯草芽孢杆菌活菌数比对照显著增了233.93%,同时显著抑制了辣椒枯萎病原菌尖孢镰刀菌专化型Ⅱ和专化型Ⅳ的生长,其菌落直径分别比对照减小5.27%和6.23%。因此,木醋液稀释250倍可促进辣椒种子萌发和抑制辣椒专化型病原菌的生长。Wood vinegar is the byproduct in activated carbon manufacture.To reuse wood vinegar in agriculture,the experiments of germination rate of chili pepper seeds and plate cultivation were conducted to explore the effects of wood vinegar on seed germination and the inhibition on pathogenic microorganisms of chili pepper.The results showed that the concentrated wood vinegar diluted for 100,250,500 times with water promoted significantly the seed germination and seedling growth of pepper when the seeds were soaked in the solution with different concentrations of wood vinegar.Meanwhile,seed germination rate,germination index,relative seedling height and root tolerance index were improved significantly compared with the control,accounting for 4.72%-10.38%,2.44%-13.06%,12.60%-39.45%and 48.50%-132.34%,respectively.The concentrated wood vinegar diluted for 250 times had the best effect on promoting the seed germination and seedling growth of chili pepper.Moreover,the concentrated wood vinegar diluted for 250 times enhanced the growth and development of beneficial bacteria significantly and the amount of Bacillus subtilis increased by 233.93%compared with the control(P<0.05).Besides,the concentrated wood vinegar diluted for 250 times inhibited fusarium oxysporum typeⅡand fusarium oxysporum typeⅣfor pepper significantly.The diameter of these two fungi reduced by 5.27%and 6.23%compared with the control(P<0.05),respectively.In summary,the concentrated wood vinegar diluted for 250 times improved the seeds germination,seedlings growth of chili pepper and inhibited the development of pathogenic microorganisms.

关 键 词：木醋液 辣椒 发芽率 抑菌效应 

分 类 号：S145.2[农业科学—肥料学] S641.3[农业科学—农业基础科学]