微阵列芯片法在抗呼吸道病原体IgM抗体检测中的应用  

The clinical application of protein microarray in the detection of serum IgM against common respiratory pathogens

作　　者：朱子菲 黎村艳 曹友德[1] 谭黎明 王启广[1] 苏娜[2] 高建业 李洋 田鑫 杨伟国[4] 张兴旺[4] 王平[4] 叶华[4] 马华瑜[4] 刘富菊 上官美荣 李琼英 李梦 何礼凯 ZHU Zi-fei;LI Cun-yan;CAO You-de;TAN Li-ming;WANG Qi-guang;SU Na;GAO Jian-ye;LI Yang;TIAN Xin;YANG Wei-guo;ZHANG Xing-wang;WANG Ping;YE Hua;MA Hua-yu;LIU Fu-ju;SHANGGUAN Mei-rong;LI Qiong-ying;LI Meng;HE Li-kai(Department of Clinical Laboratory,Translational Medicine Research Institute,The First Affiliated Hospital of Hunan Normal University(Hunan Provincial People's Hospital),Changsha 410005,China;Department of Clinical Laboratory,The First Affiliated Hospital of Lanzhou University,Lanzhou 730000,China;Changsha Weishi Medical Laboratory Co.Ltd.,Changsha 410199,China;Department of Clinical Laboratory,The People's Hospital of Gansu Province,Lanzhou 730000,China;Shenzhen Sciarray BioTech Co.Ltd.,Shenzhen 518055,China)

机构地区：[1]湖南师范大学附属第一医院(湖南省人民医院)检验科,湖南省人民医院转化医学研究所医学检验研究室,长沙410005 [2]兰州大学第一医院检验科,兰州730000 [3]长沙卫实医学检验所有限公司,长沙410199 [4]甘肃省人民医院检验科,兰州730000 [5]深圳市赛尔生物技术有限公司,深圳518055

出　　处：《现代免疫学》2021年第1期32-35,共4页Current Immunology

基　　金：湖南省科技厅技术创新引导计划临床医疗技术创新引导项目(2017TT96)。

摘　　要：为评价微阵列芯片法对呼吸道病原体IgM检测的效能及其在诊断近期呼吸道感染中的临床应用价值,以微阵列芯片法(microarray)和ELISA检测1463例疑似呼吸道病毒感染者(病例组)和806例非呼吸道病原体感染者或健康体检者(阴性对照组)的血清样本。结果显示,与ELISA相比,微阵列芯片法检测结果如下:腺病毒(adenovirus,ADV)阳性符合率为94.7%,阴性符合率为97.5%;呼吸道合胞病毒(respiratory syncytial virus,RSV)阳性符合率为94.9%,阴性符合率为97.7%;流感病毒A型(influenza virus type A,IFV-A)阳性符合率为95.3%,阴性符合率为97.9%;流感病毒B型(influenza virus type B,IFV-B)阳性符合率为93.3%,阴性符合率为97.9%;副流感病毒(parainfluenza virus,PIV)阳性符合率为93.3%,阴性符合率为97.4%;肺炎支原体(mycoplasma pneumoniae,MP)阳性符合率为94.0%,阴性符合率为98.0%;肺炎衣原体(chlamydia pneumoniae,CP)阳性符合率为93.6%,阴性符合率为97.3%。微阵列芯片法与ELISA检测急性感染期患者的阳性符合率分别为91.2%、90.3%、86.8%、88.9%、93.5%、93.8%和93.8%。由此,微阵列芯片法与ELISA结果相比具有较高的符合率。使用该方法可一次检测血清样本中7种呼吸道病原体的IgM滴度,具有操作简便、通量高、灵敏度、特异度高等优点,对近期呼吸道病原体感染的诊断和鉴别诊断有重要参考价值,值得临床推广。This study was aimed to estimate the efficacy of protein microarray technology for the detection of serum IgM against common respiratory pathogens and its clinical value in the diagnosis of recent respiratory infections.Protein microarray method was employed to detect IgM for 7 common respiratory pathogens in the serum of 1463 patients with suspected respiratory viruses infection(case group)and 806 healthy controls(negative control group).A parallel detection was undergone by ELISA,and the results from each assay were compared.The positive coincidence rate and negative coincidence rate were respectively as follows:adenovirus(ADV)(94.7%vs 97.5%),respiratory syncytial virus(RSV)(94.9%vs 97.7%),influenza virus type A(IFV-A)(95.3%vs 97.9%),influenza virus type B(IFV-B)(93.3%vs 97.9%),parainfluenza virus(PIV)(93.3%vs 97.4%),mycoplasma pneumoniae(MP)(94.0%vs 98.0%)and chlamydia pneumoniae(CP)(93.6%vs 97.3%).Compared with the control method,the microarray technology was used to detect acute infections:ADV,RSV,IFV-A,IFV-B,PIV,MP and CP positive coincidence rates were:91.2%,90.3%,86.8%,88.9%,93.5%,93.8%and 93.8%.Therefore,the protein microarray assay has a high compliance rate with ELISA.The microarray chip method can detect IgM titers of 7 respiratory pathogens in serum at one time,with high diagnostic value for respiratory virus infection.It shows that protein microarray technology has manipulation convinience,high throughput,high sensitivity and specificity,and it can be used in clinical diagnosis of recent respiratory pathogen infections.

关 键 词：呼吸道病原体 免疫球蛋白M 微阵列芯片法 酶联免疫吸附试验 

分 类 号：R446.6[医药卫生—诊断学]