约氏疟原虫感染小鼠脾脏B细胞和NK细胞及其细胞因子的检测  

Detection of B cells,NK cells and their cytokines in the spleen of Plasmodium yoelii-infected C57BL/6 mice

作　　者：陈冰霞 张梦欣 韩孟伊 张杰森 李勇森 金晨曦 齐艳伟 CHEN Bing-xia;ZHANG Meng-xin;HAN Meng-yi;ZHANG Jie-sen;LI Yong-sen;JIN Chen-xi;QI Yan-wei(The Third Clinical School,Guangzhou Medical University,Guangzhou 511436,China;Department of Pathogenic Biology and Immunology,Guangzhou Medical University,Guangzhou 511436,China)

机构地区：[1]广州医科大学第三临床学院,广州511436 [2]广州医科大学病原生物学与免疫学教研室,广州511436

出　　处：《现代免疫学》2021年第1期56-60,共5页Current Immunology

基　　金：国家自然科学基金(81902087);广东省自然科学基金(2017A030310535);广东省大学生创新创业训练计划项目(S201910570023;S201910570027);广州医科大学大学生创新创业训练项目(2018A036);广州医科大学第三临床学院大学生科研项目(2018A0020;2018A0024)。

摘　　要：为研究约氏疟原虫感染小鼠脾脏不同免疫细胞及其细胞因子的水平变化,将C57BL/6小鼠分为感染组和正常组,分别经小鼠尾静脉注射约氏疟原虫和生理盐水,8 d后处死感染组和正常组小鼠并分离脾脏,制备单细胞悬液,然后利用流式细胞术检测小鼠脾脏B细胞和NK细胞及其表达的细胞因子IFN-γ、IL-12、IL-10的水平变化情况。结果显示,感染组小鼠脾脏B细胞的百分比[(60.36±3.43)%]较正常组[(54.41±1.67)%]明显升高(P<0.05),其表达IL-12的百分比[(0.70±0.10)%]较正常组[(0.43±0.02)%]明显升高(P<0.01),表达IL-10的百分比[(1.19±0.36)%]和IFN-γ的百分比[(0.16±0.03)%]与正常组[(0.70±0.31)%、(0.13±0.03)%]比较,差异无统计学意义(P>0.05)。感染组小鼠NK细胞的百分比[(2.62±0.21)%]与正常组[(3.90±2.02)%]比较,差异无统计学意义(P>0.05),其表达IFN-γ的百分比[(49.42±4.03)%]和IL-12的百分比[(4.06±1.64)%]与正常组[(53.31±9.43)%、(4.35±1.31)%]比较,差异无统计学意义(P>0.05),而表达IL-10的百分比[(6.88±0.97)%]较正常组[(4.89±0.43)%]明显升高(P<0.05)。该研究提示,机体感染疟原虫后,小鼠的特异性免疫发挥了一定的作用,其作用途径可能是升高B细胞表达的IL-12水平,而NK细胞可能通过升高IL-10的表达水平抑制其免疫功能。The aim of this study is to investigate the changes of immune cells and cytokines in the spleen of C57 BL/6 mice infected with Plasmodium yoelii.The mice were divided into the infected group and the normal group,and the mice of the two groups were injected respectively with Plasmodium yoelii and normal saline by the tail vein.Eight days later,the mice of the two groups were sacrificed and the spleen was isolated to prepare the single cell suspension.Then the percentage of B cells,NK cells were detected by flow cytometry and the expression levels of IFN-γ,IL-12 and IL-10 were measured.The results showed that,compared with the normal group,the percentage of spleen B cells in the infected group was significantly increased[(54.41±1.67)%vs(60.36±3.43)%](P<0.05);the percentage of IL-12 was significantly increased[(0.43±0.02)%vs(0.70±0.10)%](P<0.01),while the percentages of IL-10 and IFN-γwere not significantly different from those of the normal group[IL-10,(1.19±0.36)%vs(0.70±0.31)%;IFN-γ,(0.16±0.03)%vs(0.13±0.03)%](P>0.05).There was no significant difference in the percentage of NK cells between the infected group and the nornal group[(2.62±0.21)%vs(3.90±2.02)%,P>0.05],and there was no significant difference in the percentage of IFN-γ[(49.42±4.03)%vs(53.31±9.43)%]and IL-12[(4.06±1.64)%vs(4.35±1.31)%]between the two groups as well(P>0.05).Finally,the expression level of IL-10 of the infected group was significantly higher than that of the normal group[(6.88±0.97)%vs(4.89±0.43)%](P<0.05).Taken together,these findings suggest that the specific immunity of the mice plays certain role by up-regulating IL-12 production by B cells when the body is infected with Plasmodium yoelii,whereas NK cells may inhibit the immune function by increasing the expression of IL-10.

关 键 词：约氏疟原虫 脾脏 免疫细胞 细胞因子 

分 类 号：R392.5[医药卫生—免疫学]