拟南芥GLABROUS1两个新等位突变体的筛选和鉴定  

Isolation and Characterization of Two New GLABROUS1 Alleles in Arabidopsis

作　　者：郑叶子 王丹 潘咪 王艳玲 安丽君 ZHENG Ye-zi;WANG Dan;PAN Mi;WANG Yan-ling;AN Li-jun(State Key Laboratory of Crop Stress Biology for Arid Areas,Northwest A&F University,Yangling 712100;College of Life Sciences,Northwest A&F University,Yangling 712100)

机构地区：[1]西北农林科技大学旱区作物逆境生物学国家重点实验室,杨凌712100 [2]西北农林科技大学生命科学学院,杨凌712100

出　　处：《生物技术通报》2021年第2期15-23,共9页Biotechnology Bulletin

基　　金：国家自然科学基金面上项目(31470290);中央高校基本科研业务费专项资金。

摘　　要：植物细胞命运决定机制的解析一直以来都是植物发育生物学研究的核心。模式植物拟南芥的表皮毛形成过程是研究植物细胞命运决定的优良模式系统。为了筛选和鉴定控制拟南芥表皮毛形成的新因子,我们进行了大规模的正向遗传筛选,获得了两株莲座叶表皮毛不能形成或数量显著减少的突变体f08-01和vat002-07。通过对突变基因的克隆和遗传互补实验,确定这两株突变体是GLABROUS1(GL1)的新等位突变体。GL1是调控植物表皮毛发生的关键遗传因子,其表达模式在表皮毛形成过程中受到严格调控,但是具体的机制还不清楚。通过遗传互补实验,证实GL1的3'非编码区在表皮毛形成过程中至关重要,并且3'非编码区的序列影响GL1 mRNA的稳定性和转录激活。The studies on plant developmental biology has always been centered on uncovering the underlying mechanisms of cell fate determination.Trichome in model plant Arabidopsis is an elegant system to study the molecular mechanisms of several aspects including cell fate determination of plant.To identify and screen the new genetic factors controlling the trichome initiation of Arabidopsi,we carried out the large-scale forward genetic screening,and obtained two mutants f08-01 and vat002-07 in which the trichome of rosette leaves could not be formed or the number was significantly reduced.After cloning and genetic complementation of the mutation genes,we confirmed that these two mutants represented the new alleles of GLABROUS1(GL1),which is known as a key regulator in trichome initiation.Previous studies suggested that the expression of GL1 itself might be tightly regulated during trichome development,but the underlying mechanisms are not clear yet.During our genetic complementation experiments,we found the 3'non-coding region of GL1 was vital for its function during trichome development.Further genetic and pharmacological analysis showed that the 3'non-coding sequence might be responsible for its mRNA stability and transcriptional activation.

关 键 词：表皮毛 GL1 MRNA稳定性 细胞命运决定 转录后调控 

分 类 号：Q943.2[生物学—植物学]