姜黄素通过PI3K/p53信号通路调控人胃癌细胞SGC-7901的增殖、凋亡和周期  

Curcumin regulates the proliferation, apoptosis and cycle of human gastric cancer cell SGC-7901 via the PI3K/p53 signaling pathways

作　　者：李淑兰[1] 贺倩倩[1] 宫凯凯 满玉清[1] Li Shulan;He Qianqian;Gong Kaikai(Dept of Pharmacy,Affiliated Hospital of Binzhou Medical College,Binzhou 256603;Laboratory of Cancer Reserch,Affiliated Hospital of Binzhou Medical College,Binzhou 256603)

机构地区：[1]滨州医学院附属医院药学部,滨州256603 [2]滨州医学院附属医院肿瘤研究实验室,滨州256603

出　　处：《安徽医科大学学报》2021年第1期43-48,共6页Acta Universitatis Medicinalis Anhui

基　　金：山东省医药卫生科技发展计划项目(编号:2017WS801)。

摘　　要：目的探讨姜黄素(Cur)对人胃癌细胞株SGC-7901增殖、凋亡和周期的影响及其分子机制。方法采用不同浓度梯度Cur(0、10、20、40μmol/L)处理正常培养的SGC-7901细胞。3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法检测在不同时间点(24、48、72 h)细胞增殖能力变化;Hoechst 33258荧光染色观察细胞凋亡状况;流式细胞术检测细胞凋亡水平及周期变化;Western blot检测PI3K/p53信号通路相关蛋白表达变化。结果 MTT结果显示,与对照组比较,Cur呈浓度依赖性抑制SGC-7901细胞增殖;细胞荧光染色结果显示Cur处理组的细胞亮蓝色荧光强度减弱;流式细胞术结果显示,与对照组比较,不同浓度Cur处理组细胞凋亡率增加(P<0.05,P<0.01),G0/G1期细胞出现阻滞(P<0.01);Western blot结果显示,与对照组比较,不同浓度Cur组细胞PI3K蛋白表达量、Akt磷酸化水平均降低(P<0.01),而p21和p53蛋白表达量升高(P<0.05,P<0.01)。结论 Cur能抑制SGC-7901细胞的增殖效应,通过诱导细胞发生G0/G1期阻滞进而促进其凋亡进程,这可能与其对PI3K/p53信号通路的调控密切相关。Objective To investigate the effect of Curcumin(Cur) on the proliferation, apoptosis and cycle of human gastric cancer cell line SGC-7901 and its molecular mechanism.Methods SGC-7901 cells were treated with different concentration of Cur(0,10,20,40 μmol/L).3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay was used to detect the changes in cell proliferation at different time points(24,48,72 h).Hoechst 33258 fluorescence staining was used to observe cell status of apoptosis.Flow cytometry was used to detect apoptosis and cell cycle.However,the changes of related protein expression in PI3K/p53 signaling pathway were detected by Western blot.Results MTT results showed that the proliferation of SGC-7901 cells was inhibited by Cur in concentration-dependent manners.The results of cell fluorescence staining showed that Cur could promote the apoptosis of SGC-7901 cells,and the bright blue fluorescence intensity of cells reduced.The results of flow cytometry showed that compared with the control group,the apoptosis rate increased(P<0.05,P<0.01) and G0/G1 phase cell was blocked in the group with different concentrations of Cur groups(P<0.05).Western blot results showed that the protein level of PI3K and the phosphorylation level of Akt in cells of different concentrations reduced compared with the control group(P<0.01),but the expression levels of p21 and p53 increased(P<0.05,P<0.01).Conclusion Cur inhibits the proliferation of SGC-7901 cell,induce G0/G1 phase arrest and promote apoptosis,which may be closely related to the regulation of PI3K/p53 signaling pathways.

关 键 词：姜黄素 人胃癌细胞 增殖 凋亡 PI3K/p53信号通路 细胞周期 

分 类 号：R285.5[医药卫生—中药学] R735.2[医药卫生—中医学]