羊毛固醇合成酶抑制剂RO对KCs细胞增殖的影响  

Effects of lanosterol synthase inhibitor RO on proliferation of keratinocytes

作　　者：刘莉 顾亚男 周宏 李名聪 罗欣 张胜权 Liu Li;Gu Yanan;Zhou Hong(Center for Scientific Research of Anhui Medical University,Hefei 230032)

机构地区：[1]安徽医科大学科研实验中心,合肥230032

出　　处：《安徽医科大学学报》2021年第2期176-179,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81271748)。

摘　　要：目的研究羊毛固醇合成酶抑制剂(RO)单独或联合胆固醇(CH)使用对皮肤角质形成细胞(KCs)细胞增殖及细胞周期的影响。方法 RO不同剂量作用KCs一定时间,采用MTS比色法检测KCs细胞增殖的变化;使用一定浓度的RO单独或联合CH作用KCs不同时间,使用MTS比色法和流式细胞仪检测记录KCs细胞增殖和细胞周期的变化;单独使用相同浓度RO或联合CH经过不同时间刺激KCs后通过蛋白质免疫印迹法对细胞周期蛋白CyclinB1、CyclinE表达水平进行分析。结果 RO抑制KCs细胞增殖,并呈浓度和时间依赖性;联合使用CH降低RO对KCs的增殖抑制作用;RO可时间依赖性增加KCs细胞周期在G1期比例,联合使用CH缓慢增加KCs周期在G1期比例;RO可时间依赖性降低细胞周期调控蛋白CyclinB1、CyclinE的表达,联合使用CH部分拮抗RO下调CyclinE,并具有时间效应,而CyclinB1的表达没有受到明显的影响。结论 RO可能通过影响CyclinB1、CyclinE的表达使KCs细胞周期G1期阻滞进而抑制KCs增殖。Objective To investigate the effect of RO or combined cholesterol(CH) on keratinocytes cell proliferation and cyclin expression.Methods The KCs were cultured with different concentrations of RO for different times,the proliferation of KCs were analyzed by MTS.Cell cycle of KCs were detected by flow cytometry after using RO with different times or combined treated with CH,and Western blot was used to reveal the expression of CyclinB1 and CyclinE.Results The RO inhibited the proliferation of KCs in a quantitative and time-dependent manner.Combined use of CH reduced the proliferation inhibition of RO on KCs.RO could significantly increase the proportion of KCs cell cycle in G1 phase,while combined use of CH reduced G1 phase growth rate of KCs cell cycle.Western blot demonstrated that use of RO could reduced the CyclinB1 and CyclinE content in the KCs in a time dependence manner.Combined use of CH antagonism RO down-regulated Cyclin E,but had no significant effect on the expression of CyclinB1.Conclusion RO inhibits the proliferation of KCs by down-regulating the expression of CyclinB1 and CyclinE which blocks the G1 phase of KCs cell cycle.

关 键 词：RO CH KCs 细胞增殖 细胞周期蛋白 

分 类 号：R3[医药卫生—基础医学]