迟缓埃格特菌的MALDI-TOF MS和16S rRNA鉴定及生物学特征研究  

Identification of MALDI-TOF MS and 16S rRNA gene and biological characteristics of Eggerthella lenta

作　　者：李祥云 王中新[1] 朱帮强[2] 潘亚萍[1] 周寅第 胡媛媛[1] 宋国滨 徐元宏[1] Li Xiangyun;Wang Zhongxin;Zhu Bangqiang(Dept of Laboratory Medicine,The First Affiliated Hospital of Anhui Medical University,Hefei 230022;Dept of Blood Transfusion,The First Affiliated Hospital of Anhui Medical University,Hefei 230022)

机构地区：[1]安徽医科大学第一附属医院检验科,合肥230022 [2]安徽医科大学第一附属医院输血科,合肥230022

出　　处：《安徽医科大学学报》2021年第2期191-194,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81171606);安徽医科大学第一附属医院博士科研启动基金(编号:BSKY2019038)。

摘　　要：目的鉴定两株迟缓埃格特菌临床分离株。方法选取两株厌氧血培养瓶培养出的分离菌株,采用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)和革兰染色鉴定分离株,药敏实验采用琼脂稀释法。同时提取菌株基因组DNA,选用16S rRNA通用引物进行PCR扩增,回收和纯化产物后进行测序,与GeneBank数据库中已知菌序列进行比对,并构建系统发育树。结果 MALDI-TOF MS与16S rRNA基因检测均鉴定分离株是迟缓埃格特菌。药敏实验显示该菌对青霉素、哌拉西林-他唑巴坦、氨苄西林-舒巴坦、亚胺培南、美罗培南、甲硝唑和克林霉素敏感。结论 MALDI-TOF MS和16S rRNA检测可用于迟缓埃格特菌等罕见菌的鉴定。Objective To identify two clinical isolate strains of Eggerthella lenta.Methods Two strains isolated from positive blood anaerobic culture were selected.The isolates were identified by MALDI-TOF MS and gram staining.Drug sensitivity test was performed by agar dilution method.At the same time,genomic DNA of the strains was extracted,16 S rRNA universal primer was used for PCR amplification,and the product was sequenced after recovery and purification,which was homologous with the sequences of the known bacteria in GeneBank database.Results The isolate was identified as Eggerthella lenta by MALDI-TOF MS and 16 S rRNA gene assay.Drug sensitivity tests showed that the bacteria was sensitive to penicillin,piperacillin-tazobactam,ampicillin-sulbactam,imipenem,meropenem,metronidazole and clindamycin.Conclusion MALDI-TOF MS and 16 S rRNA detection can be used for identification of rare bacteria such as Eggerthella lenta.

关 键 词：迟缓埃格特菌 MALDI-TOF MS 16S rRNA 药敏实验 

分 类 号：R446.5[医药卫生—诊断学]