芍药苷-6′-O-苯磺酸酯对高胰岛素诱导足细胞功能损伤的影响  

Effect of paeoniflorin-6′-O-benzene sulfonate on podocyte function injury induced by high insulin

作　　者：黄琼芳 赵天娇 苏扬妮 黄琼[1] 魏伟[1] Huang Qiongfang;Zhao Tianjiao;Su Yangni(Institute of Clinical Pharmacology,Anhui Medical University,Key Laboratory of Anti-Inflammatory and Immune Medicine,Ministry of Education,Collaborative Innovation Center of Anti-Inflammatory and Immune Medicines,Hefei 230032)

机构地区：[1]安徽医科大学临床药理研究所,抗炎免疫药物教育部重点实验室,抗炎免疫药物安徽省协同创新中心,合肥230032

出　　处：《安徽医科大学学报》2021年第2期267-271,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81974508、81673492)。

摘　　要：目的探讨芍药苷-6′-O-苯磺酸酯(CP-25)对高胰岛素诱导足细胞损伤的保护作用及机制。方法体外培养小鼠足细胞,分为空白对照组、高胰岛素组、高胰岛素+CP-25(0.1、1、10μmol/L)组、高胰岛素+二甲双胍组,分别处理足细胞。CCK-8法检测CP-25对高胰岛素诱导的足细胞增殖的影响;GOD-POD法检测CP-25对高胰岛素诱导的足细胞葡萄糖消耗的影响;Transwell法检测CP-25对高胰岛素诱导的足细胞迁移的影响;Annexin V-FITC/PI双染法检测CP-25对高胰岛素诱导的足细胞凋亡的影响;Western blot法检测CP-25对高胰岛素诱导的足细胞Nephrin蛋白表达的影响。结果 CCK-8结果显示:CP-25能够增加高胰岛素诱导的足细胞的增殖能力;GOD-POD结果显示:CP-25能够改善高胰岛素诱导的足细胞对葡萄糖的摄取能力;Transwell实验结果显示:CP-25能够减少高胰岛素诱导的足细胞的迁移;Annexin V-FITC/PI双染结果显示:CP-25能够抑制高胰岛素诱导足细胞的凋亡;Western blot结果显示:CP-25能够上调高胰岛素诱导的足细胞Nephrin蛋白表达。结论 CP-25能够改善高胰岛素诱导的足细胞的损伤,其机制可能与提高足细胞Nephrin蛋白的表达有关。Objective To investigate the protective effect and mechanism of CP-25 on high insulin-induced podocyte injury.Methods Mouse podocyte were cultured in vitro and divided into blank control group,high-insulin model group,high insulin+CP-25(0.1、1、10 μmol/L) group and Metformin group.The effect of CP-25 on the proliferation of podocyte induced by high insulin was detected by CCK-8 method.The effect of CP-25 on glucose consumption induced by high insulin in podocyte was detected by GOD-POD method.Transwell method was used to detect the effect of CP-25 on the migration of podocyte induced by high insulin.Annexin V-FITC/PI double staining assay was used to detect the effect of CP-25 on high insulin induced apoptosis of podocyte.The effect of CP-25 on the expression of Nephrin protein in podocyte induced by high insulin was detected by Western blot.Results CCK-8 results showed that CP-25 could increase the proliferation capacity of high insulin induced podocyte.The results of GOD-POD showed that CP-25 could improve the glucose uptake ability of high insulin induced podocyte.Transwell experimental results showed that CP-25 could reduce the migration of podocyte induced by high insulin.Annexin V-FITC/PI double staining results showed that CP-25 could inhibit the apoptosis of pdocyte induced by high insulin.Western blot results showed that CP-25 could up-regulate the protein expression of Nephrin in podocyte induced by high insulin.Conclusion CP-25 can improve the injury of podocyte induced by high insulin,and the mechanism may be related to the expression of Nephrin.

关 键 词：足细胞 CP-25 胰岛素 NEPHRIN 

分 类 号：R587[医药卫生—内分泌]