高端电子显微镜实验室环境设计与建设技术要点  

Key points of environmental design and construction of high-end electron microscope laboratory

作　　者：郭振玺 张斌[3] 豆瑞发[4] 茶丽梅 陈永圣[6] 邵博 裴霞[1,2] 韩玉刚 GUO Zhen-xi;ZHANG Bin;DOU Rui-fa;CHA Li-mei;CHEN Yong-sheng;SHAO Bo;PEI Xia;HAN Yu-gang(School of Life Science,Beijing 100871;Cryo EM core facility Peking university,Beijing 100871;Analytical and testing center of chongqing university,Chongqing 401331;Norendar international LTD,Shijiazhuang Hebei 050011;Material department of Guangdong technion israel institute of technology,Shantou Guangdong,515063;Core Facility for Protein Research,Chinese Academy of Sciences,Beijing 100101,China)

机构地区：[1]北京大学生命科学学院,北京100871 [2]北京大学冷冻电镜平台,北京100871 [3]重庆大学分析测试中心,重庆401331 [4]北方工程设计研究院有限公司,河北石家庄050011 [5]广东以色列理工学院材料系,广东汕头515063 [6]中国科学院生物物理研究所蛋白质科学研究平台,北京100101

出　　处：《电子显微学报》2021年第1期78-89,共12页Journal of Chinese Electron Microscopy Society

基　　金：北京大学仪器创新研制项目(No.6202000080/003);北京航空航天大学工程训练中心合作项目(No.8300300194);深圳军民融合装备技术研究院合作项目(No.8430102318).

摘　　要：电子显微镜(以下简称电镜)是具有超高分辨率的高精密电子光学仪器,广泛应用于科研、工业、医疗、食品安全和生命健康等众多领域,已成为现代物质形态与微结构的重要测试表征与科学研究仪器。近年来,随着球差/色差校正技术、各类原位电镜技术等的快速发展与应用,原子尺度(静态与动态)微结构图像的获取已不再遥不可及。冷冻电镜的发展与普及,更是为生物大分子复合物、软物质等的研究带来了革新。相对于性能指标的极大提升,高端电镜对于运行环境的要求亦越发严苛。以冷冻电镜解析大分子复合物结构为例,单次数据收集时间往往需要数天,因此环境指标及其稳定性对设备的运行至关重要。如何保证电镜稳定的运行,以获得连续的高质量图像、降低设备故障率、提高利用率、减少使用成本等问题,已成为电镜管理人员和使用者面临的首要问题。总之,稳定的环境是电镜良好运行的必要保障,环境磁场、振动、噪音、温度及其波动、湿度、独立接地、电源与附属机房等问题均会对电镜的性能与稳定运行产生重要影响,这也是电镜室环境改造的重要指标。本文将结合作者自身的电镜室环境改造些许经验,简略探讨高端电镜环境改造的技术要点,以期能为广大同行、用户提供一定参考与借鉴。As a high-precision electronic optical instrument with ultra-high resolution, electron microscope(EM) has been widely used in the scientific research, including industry, medical care, food safety, life health, and many other fields, and becoming an important tool for conducting testing, characterization as well as scientific research on the modern material form and microstructure. In recent years, with the rapid development and application of the spherical/chromatic aberration corrector and the state-of-the-art in-situ EM technology, it is no longer so difficult to obtain atomic-scale(static or dynamic) micro-structure images. Moreover, the development and popularity of Cryo-electron microscopy has brought innovation to the study of biomacromolecules, soft matter, etc. Associated with the great performance improvement of EM, the requirements for the operating environment become even higher. For instance, it usually takes several days to perform data collection in a single case regarding the structure analysis of macromolecular complexes using cryo-TEM, which raises stricter requirements for the EM stability. Therefore, environmental index and its stability are crucial to the device operation. Now, it is a priority for the EM workers/users to build a stable condition for EM, particularly the high-end EM, in order to continuously obtain high-quality images, improve the utilization rate of the EM, and decrease equipment failure rate as well as the cost. Generally, the stable environment is the foundation and prerequisite for the high-performance achievement of EM. Environmental magnetic field, vibration, temperature and its fluctuation, humidity, independent grounding, supply systems(e.g., electron power and cooling water) and auxiliary machine room and others factors will affect the stable operation and performance of EM significantly. These factors are also key performance evaluations for environmental improvements. In this paper, based on the authors’ relevant experience, some key points for the environmental

关 键 词：冷冻 电子显微镜 环境改造 磁屏蔽 独立接地 

分 类 号：TN16[电子电信—物理电子学] TB535.1[理学—物理]