基于“寒凝血瘀”关键病机理论探讨新加良附方治疗胃癌的网络药理学研究  

Based on the Key Pathogenesis Theory of“Cold Coagulation and Blood Stasis”Discussing the Network Pharmacology Study of Modified Liang-Fu Formula In The Treatment of Gastric Cancer

作　　者：国佳鑫 侯丽[1] 蔡和平 栗枭杰 杨茜茹 张思亲 董青[1] GUO Jiaxin;HOU Li;CAI Heping;LI Xiaojie;YANG Qianru;ZHANG Siqin;DONG Qing(Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100020,China;Beijing Traditional Chinese Medicine Hospital Shunyi Hospital,Beijing 101399,China;Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China)

机构地区：[1]北京中医药大学东直门医院,北京100020 [2]北京中医医院顺义医院,北京101399 [3]成都中医药大学附属医院,成都610075

出　　处：《世界中医药》2021年第3期373-380,共8页World Chinese Medicine

基　　金：首都卫生发展科研专项项目(2020-2-4193)--基于中医肿瘤注册登记平台对晚期胃癌中西并重治疗方案的研究与优化。

摘　　要：目的:通过网络药理学方法探讨新加良附方治疗胃癌的作用机制。方法:在中药系统药理学数据库与分析平台(TCMSP)中获取高良姜、香附、穿山龙的有效成分及靶蛋白,通过Genecards、OMIM数据库筛选出胃癌的靶点,利用Venn软件获取共同靶点,使用Cytoscape3.7.2进行“化合物-靶点-疾病”的网络图构建。通过String数据库获取蛋白互作网络(PPI),最后进行GO功能、KEGG通路富集分析。结果:从新加良附方中筛选出高良姜素、槲皮素、木犀草素、薯蓣皂苷元等主要有效活性成分,作用于87个胃癌靶点,筛选出AKT1、MYC、CASP3、EGFR等关键靶点,并确定了PI3K-Akt信号通路、p53信号通路、Wnt信号通路等关键代谢信号通路,参与蛋白结合、转录因子活性、酶活性调节、受体活性等过程。结论:新加良附方通过多个靶点、多条通路发挥治疗胃癌的作用。Objective:To explore the mechanism of action of modified Liang-Fu Formula in the treatment of gastric cancer through network pharmacology method.Methods:The effective components and target proteins of Alpinia officinarum Hance,Cyperus rotundus L and Discorea nipponica Makino were from the traditional Chinese medicine system pharmacology analysis platform(TCMSP).And the target of gastric cancer was gotten through the Genecards and OMIM database.Venn online software was used to obtain the common target,and the“compound-target-disease”network diagram was constructed by using Cytoscape 3.7.2 software.The String database was used to draw the protein interaction(PPI)network.Finally,enrichment analysis of GO function and KEGG pathway was carried out.Results:The main active components,such as galangin,quercetin,luteolin and diosgenin,were screened out from the negaeliang side,and acted on 87 gastric cancer targets.Key targets,such as AKT1,MYC,CASP3 and EGFR,were screened out,and key metabolic signaling pathways,such as pi3K-Akt signaling pathway,p53 signaling pathway,and Wnt signaling pathway,were determined.It was involved in protein binding,transcription factor activity,enzyme activity regulation,receptor activity and other processes.Conclusion:Modified Liangfu Formula plays a role in the treatment of gastric cancer through multiple targets and pathways.

关 键 词：新加良附方 寒凝血瘀 高良姜 香附 穿山龙 胃癌 网络药理学 作用机制 

分 类 号：R285[医药卫生—中药学]