不同产地酒女贞子红景天苷、特女贞苷含量测定及聚类分析  

Determination of Salidroside and Nuezhenide in Different Producing Areas and Cluster Analysis

作　　者：张柏生[1] 李斌[1] 李茜[1] 于浩楠 李伟[1] ZHANG Bosheng;LI Bin;LI Qian;YU Haonan;LI Wei(Affiliated Chinese Medicine Hospital of Xinjiang Medical University,Urumqi 830000,China;Xinjiang Medical University College of Traditional Chinese Medicine,Urumqi 830011,China)

机构地区：[1]新疆医科大学附属中医医院,乌鲁木齐830000 [2]新疆医科大学中医学院,乌鲁木齐830011

出　　处：《世界中医药》2021年第3期426-429,共4页World Chinese Medicine

基　　金：新疆医科大学附属中医医院院级课题炮制专项(ZYY2017PZ7)。

摘　　要：目的:研究不同产地酒女贞子红景天苷和特女贞苷含量,为女贞子饮片质量标准提供依据。方法:采用HPLC测定不同产地酒女贞子红景天苷和特女贞苷含量,运用聚类分析法,结合SPSS统计软件对测定结果进行综合分析。比较不同产地女贞子炮制前后含量变化。结果:通过高效液相色谱测定得到红景天苷在0.1~0.5 mg范围内呈良好的线性关系,红景天苷的回归方程为y=23961113.0000 x-219608.5000,R^(2)=0.9992,平均加样回收率为100.98%,RSD=3.46%(n=6);通过高效液相色谱测定得到特女贞苷在50~250μg范围内呈良好的线性关系,特女贞苷的回归方程为y=214086.65200 x-5991365.0000,R^(2)=0.9997,平均加样回收率为100.87%,RSD=2.42%(n=6),采用聚类分析可分为三类,其中第三类的成分含量综合评分排名较前,说明该类地区酒制女贞子质量较好。女贞子炮制后,红景天苷和特女贞苷均有明显的提高。结论:聚类分析具有方法简便,精密度高,可预测性较好的优点,可用于中药饮片质量分析。Objective:To study the content of salidroside and nuezhenide in Ligustrum lucidum from different habitats,and to provide evidence for the standard of Ligustrum lucidum.Methods:The contents of salidroside and nuezhenide were determined by HPLC.The results of cluster analysis were combined with SPSS software to analyze the results.The changes of the content of Ligustrum lucidum from different producing areas before and after processing were compared.Results:The linearity of salidroside in the range of 0.1~0.5 mg was determined by high performance liquid chromatography.The regression equation of salidroside was y=23961113.0000 x-219608.5000,r^(2)=0.9992,and the average recovery rate was 100.98%,RSD=3.46%(n=6);the high-performance liquid chromatographic determination of nuezhenide showed a good linear relationship in the range of 50~250μg.The regression equation of nuezhenide was y=214086.65200 x-5991365.0000,r^(2)=0.9997,the average recovery rate was 100.87%,RSD=2.42%(n=6),which can be divided into 3 categories by cluster analysis.The third category had higher comprehensive scores,indicating that the quality of the wine cellars in the region was good.After the preparation of Ligustrum lucidum,salidroside and nuezhenide had been significantly improved.Conclusion:Cluster analysis has the advantages of simple method,high precision and good predictability and it can be used for Chinese medicine decoction pieces.

关 键 词：女贞子 HPLC 聚类分析 含量测定 红景天苷 特女贞苷 炮制 质量评价 

分 类 号：R284.2[医药卫生—中药学] R917[医药卫生—中医学]