嗜酸性粒细胞阳离子蛋白在小儿迁延性、慢性腹泻病患儿中的表达  

Expression of Eosinophil Cationic Protein in Children with Persistent and Chronic Diarrhea

作　　者：陈红苓 薛宁[1] 徐俊杰[1] 孟娇[1] CHEN Hongling;XUE Ning;XUN Junjie;MENG Jiao(Jinan Children's Hospital,Jinan,Shandong 250022,China)

机构地区：[1]济南市儿童医院,山东济南250022

出　　处：《大医生》2020年第22期27-29,共3页Doctor

基　　金：济南市科学技术发展计划(编号:201704119)。

摘　　要：目的探讨嗜酸性粒细胞阳离子蛋白(ECP)在无明确原因、无血便的小儿迁延性、慢性腹泻患儿血清及肠黏膜中的表达。方法选取2017年1月至2019年6月在济南市儿童医院住院的迁慢性腹泻40例患儿为患儿组,另选择40例正常查体儿童为血清对照组,以及40例接受先天性巨结肠肠切除术后近端正常的患儿作为黏膜对照组。分别用酶联免疫吸附试验(ELISA)法测定血清对照组及患儿组血清ECP水平;免疫组化方法测定患儿组及黏膜对照组中ECP水平。结果患儿组血清ECP升高明显,与血清组相比差异有统计学意义(P<0.01),而患儿组仅3例见ECP呈阳性的嗜酸性粒细胞脱颗粒现象,与黏膜对照组相比差异无统计学意义(P>0.05)。结论目前不能明确ECP参与小儿迁延性慢性腹泻的发病。Objective To investigate the expression of eosinophil granulocyte cationic protein(ECP)in serum and intestinal mucosa of children with persistent and chronic diarrhea without hematochezia and without definite cause.Methods 40 children with chronic diarrhea who were admitted to Jinan Children's Hospital from January 2017 to June 2019 were selected as the patients group,and 40 normal children were selected as the serum control group,40 children with normal proximal end after hirschsprung's resection were served as the mucosa control group.ELISA method was used to determine serum control group and patients group,immunohistochemical method was used by patients group and mucosa control group.Results The results showed that there were significant differences in patients group(P<0.01),but only 3 cases of ECP positive eosinophil degranulation were found in the patients group,and the difference was not statistically significant(P>0.05).Conclusion ECP is not involved in the pathogenesis of persistent chronic diarrhea in children.

关 键 词：儿童 迁延性、慢性腹泻 嗜酸性粒细胞阳离子蛋白 

分 类 号：R72[医药卫生—儿科]