Unraveling independent origins of two tetraploid Achillea species by amplicon sequencing  

作　　者：Jun-Nan Wan Yan-Ping Guo Guang-Yuan Rao 

机构地区：[1]School of Life Sciences,Peking University,Beijing 100871,China [2]Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering,and College of Life Sciences,Beijing Normal University,Beijing 100875,China

出　　处：《Journal of Systematics and Evolution》2020年第6期913-924,共12页植物分类学报（英文版）

基　　金：the National Natural Science Foundation of China(Grant Nos.31670221 and 31570215).

摘　　要：Allopolyploidy is a significant mechanism of plant speciation,and many allopolyploid species have arisen recurrently.However,the probability that allopolyploidization between the same two parental species could lead to the origin of different taxa has received little attention.Here we used a new progenitor-specific amplicon sequencing method to demonstrate the independent origins of two yarrow species,Achillea alpina L.and Achillea wilsoniana Heimerl ex Hand.-Mazz.,through allotetraploidy from the same diploid progenitor species pair,Achillea acuminata(Ledeb.)Sch.Bip.and Achillea asiatica Serg.Based on the sequences of 17 nuclear genes from 21 wild populations of the four Achillea species investigated,a clear view of genetic structure and demographic history was obtained with each species.Significant genetic differentiation was evident between the two tetraploid species.Two genetically distinguishable groups were detected within one of the progenitor,A.acuminata,and ancestors belonging to those two groups contributed to the two tetraploid species.Excluding fixed heterozygosity,we detected extremely low genetic diversity in many populations of both tetraploid species.Approximate Bayesian computation indicated that both tetraploid species originated before the Last Glacial Maximum,and nearly all diploid lineages went through population declines after the allopolyploidization events.Our study indicates that independent allopolyploidization events between the same Achillea parental species have generated two genetically and ecologically distinct taxa.

关 键 词：ACHILLEA allopolyploid speciation amplicon sequencing demographic history population genetic structure 

分 类 号：Q94[生物学—植物学]