虎杖象甲培植真菌营养特性及对菌丝附属物形成的影响  

Nutritional requirements and their effects on the formation of hyphal surface appendages of symbiotic fungus cultivated by attelabid weevil

作　　者：韩一多 向梅春[2] 刘杏忠 HAN Yi-Duo;XIANG Mei-Chun;LIU Xing-Zhong(College of Life Sciences,University of Science and Technology of China,Hefei,Anhui 230027,China;State Key Laboratory of Mycology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China;College of Life Sciences,Nankai University,Tianjin 300071,China)

机构地区：[1]中国科学技术大学生命科学学院,安徽合肥230027 [2]中国科学院微生物研究所真菌学国家重点实验室,北京100101 [3]南开大学生命科学学院,天津300071

出　　处：《菌物学报》2020年第12期2268-2276,共9页Mycosystema

基　　金：国家自然科学基金(31270070)。

摘　　要：虎杖象甲培植共生真菌形成的共生体系是植菌昆虫菌业中的典型代表。共生真菌Penicillium herquei如何向虎杖象甲Euopschinensis提供营养尚未明确。本研究发现共生真菌P.herquei的菌丝表面存在大量瘤状凸起物及由凸起物衍生的附属丝等特化结构,该结构可能为虎杖象甲提供营养;对共生真菌的营养研究表明,共生真菌能高效利用山梨醇、蔗糖、海藻糖、葡萄糖等单糖或双糖,以及酪氨酸、甘氨酸、谷氨酰胺等昆虫非必须氨基酸,同时在高碳和最适碳源条件下有利于菌丝特化附属物的产生。研究结果不仅提供了植菌卷叶象甲菌业中共生真菌在营养方面的适应性进化证据,而且为进一步揭示共生真菌适应卷叶象甲的营养机制奠定了基础。Association between Euops chinensis and its symbiotic fungus Penicillium herquei is a typical attelabid fungiculture.How P.herquei provides nutrition for E.chinensis is unknown.We found that there were abundant appendages of nodular protrusions and networks on the hyphal surface of P.herquei,which might be the nutrient for host weevil.The nutritional requirements of P.herquei indicated mono-and di-saccharides such as glucitol,sucrose,trehalose,and glucose as carbon sources and non-essential amino acids of insect such as tyrosine,glycine,and glutamine as nitrogen sources supported the well growth of P.herquei.High-carbon and optimal carbon source environments can promote production of more appendages on the hyphal surface.The results not only provide evidence of symbiotic fungus nutritional adaptation in the attelabid weevil fungiculture,but also lay the foundation for further revealing the nutritional mechanism of the symbiotic fungus adapting to the leaf rolling weevil.

关 键 词：植菌昆虫 共生互作 特化结构 适应性进化 营养机制 

分 类 号：Q948.122.2[生物学—植物学]