基于网络药理学的丹七片治疗冠心病作用机制研究  

The mechanism of Danqi tablets in the treatment of coronary heart disease based on network pharmacology

作　　者：马林 邵明燕 孙乾斌 李春 王勇 MA Lin;SHAO Ming-yan;SUN Qian-bin;LI Chun;WANG Yong(School of Life Science,Beijing University of Chinese Medicine,Beijing 100029,China;School of Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China;School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学生命科学学院,北京100029 [2]北京中医药大学中药学院,北京100029 [3]北京中医药大学中医学院,北京100029

出　　处：《药学学报》2020年第12期2942-2950,共9页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(81822049,81673712).

摘　　要：利用网络药理学方法探究丹七片治疗冠心病的活性成分和作用机制,为临床冠心病的治疗提供理论依据。采用TCMSP数据库筛选出丹七片的活性成分及其靶标;通过GeneCards数据库筛选冠心病的预测靶点;将两者靶点进行映射交集;利用STRING数据库构建蛋白互作网络图并筛选出65个核心靶点;再通过DAVID数据库进行GO(gene ontology)生物过程和KEGG信号通路富集分析,最后利用Cytoscape-3.6.2软件构建活性成分-关键靶点-作用通路网络图。通过建立过氧化氢(H2O2)诱导的心肌细胞H9C2损伤模型,对关键活性成分进行实验验证。网络药理学研究提示,丹七片通过槲皮素、木犀草素和丹参酮IIA等活性成分作用于炎症因子和细胞凋亡相关通路,改善体内促炎或抗炎失衡,保护心肌细胞,从而达到治疗冠心病的目的,证实了丹七片治疗冠心病多成分、多靶点的作用特点,为其进一步的实验研究及临床诊治提供客观依据。We used the network pharmacology to explore the active ingredients and mechanism of action of Danqi tablets in the treatment of coronary heart disease,providing a theoretical basis for the treatment of clinical coronary heart disease.The TCMSP database was used to screen for active ingredients and targets in Danqi tablets;the predicted targets of coronary heart disease were screened through the GeneCards database;then,the intersection of the two targets was mapped;we used STRING database to construct a protein interaction network map and identified 65 cores targets;then,the DAVID database was used for enrichment analysis of gene ontology(GO)biological processes and KEGG signaling pathways,and finally the Cytoscape-3.6.2 software was used to construct a network diagram of traditional Chinese medicine-active ingredient-key targets-pathway.The H2O2-induced H9C2 cells injury model was used for experimental verification.The results suggest that Danqi tablets act on inflammatory factors and apoptosis-related pathways through quercetin,luteolin,tanshinone IIA,and other active ingredients,and improve proinflammatory or anti-inflammatory imbalances in the body and protect cardiomyocytes.This study confirms the multi-component and multi-target effects of Danqi tablets in the treatment of coronary heart disease,and provides an objective basis for their use in further experimental research and the clinical diagnosis and treatment of coronary heart disease.

关 键 词：网络药理学 丹七片 冠心病 作用机制 靶点 

分 类 号：R966[医药卫生—药理学]