皂角刺中的一个新木脂素  

A new lignan from Gleditsiae spina

作　　者：何晨 曹彦刚 齐曼[1,2] 张贝贝 任英杰 刘晏灵 王梦娜 郑晓珂 冯卫生[1,2] HE Chen;CAO Yan-gang;QI Man;ZHANG Bei-bei;REN Ying-jie;LIU Yan-ling;WANG Meng-na;ZHENG Xiao-ke;FENG Wei-sheng(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;The Engineering and Technology Center for Chinese Medicine Development of Henan Province China,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学药学院,河南郑州450046 [2]河南省中药开发工程技术研究中心,河南郑州450046

出　　处：《药学学报》2020年第12期2951-2954,共4页Acta Pharmaceutica Sinica

基　　金：中央引导地方科技重大发展专项:河南道地大宗药材种质评价及集约化种植与示范(豫财科[2016]149号).

摘　　要：利用MCI gel CHP-20、ODS、Sephadex LH-20、硅胶及半制备高效液相等多种色谱技术,从皂角刺乙酸乙酯部位分离得到7个木脂素类化合物,并根据波谱数据分析鉴定其结构,分别为(7R,8S,7'E,7''S,8''R)-buddlenol P(1)、(+)-丁香脂素(2)、(+)-异落叶松脂素(3)、(7S,8R)-cedrusin(4)、(7S,8R)-4,9,9'-三羟基-3,3'-二甲氧基-7,8-二氢苯骈呋喃-1'-丙基新木脂素(5)、3',4-O-dimethylcedrusin(6)和蛇菰宁(7)。其中化合物1为新化合物,命名为(7R,8S,7'E,7''S,8''R)-buddlenol P,化合物2~7为首次从皂荚属中分离得到。采用MTT法探究化合物2~7对脂多糖(LPS)诱导的大鼠肾小管上皮细胞损伤的干预作用,化合物2、3和7对LPS诱导的NRK-52e细胞损伤具有保护作用。The chemical constituents from ethyl acetate extract of Gleditsiae spina were isolated and purified by various chromatographic methods such as MCI gel CHP-20,ODS,Sephadex LH-20,silica gel and semi-preparative HPLC.Seven lignans were isolated and identified by spectroscopic data analyses as(7R,8S,7'E,7''S,8''R)-buddlenol P(1),(+)-syringaresinol(2),(+)-isolariciresinol(3),(7S,8R)-cedrusin(4),(7S,8R)-4,9,9'-trihydroxy-3,3'-dimethoxy-7,8-dihydrobenzofuran-1'-propylneolignan(5),3',4-O-dimethylcedrusin(6),balanophonin(7).Among them,compound 1 is a new lignan,compounds 2-7 are isolated from the Gleditsia L.for the first time.MTT method was used to investigate the effect of compounds 2-7 on LPS-induced injury of NRK-52e cells.As a result,compounds 2,3 and 7 exhibit protective effects against LPS-induced damage to NRK-52e cells.

关 键 词：皂荚属 皂角刺 木脂素类 (7R 8S 7'E 7''S 8''R)-buddlenol P 

分 类 号：R284[医药卫生—中药学]