分子标记辅助选择改良玉米自交系昌7-2的丝黑穗病抗性  

Improving Head Smut Resistance in Maize Inbred Line Chang7-2 by Marker Assisted Selection

作　　者：邸宏[1] 宫程旭 孙培元[1] 刘显君[1] 王振华[1] DI Hong;GONG Cheng-xu;SUN Pei-yuan;LIU Xian-jun;WANG Zhen-hua(Agricultural College,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北农业大学农学院/教育部寒地粮食作物种质创新与生理生态重点实验室,哈尔滨150030

出　　处：《玉米科学》2021年第1期20-25,共6页Journal of Maize Sciences

基　　金：国家自然科学基金项目(31971956)。

摘　　要：选取5个与bin2.09主效抗病位点紧密连锁的分子标记,利用含主效抗病位点的黄早四近等基因系1JD006为供体亲本,开展连续回交分子标记辅助选择育种,定向改良玉米自交系昌7-2的丝黑穗病抗性。结果表明,标记3M1-25选择效率最高,其次为标记MZA6393。结合分子标记辅助选择和田间接种鉴定,在BC2F2群体中筛选出背景回复率大于理论值87.50%的6个单株,BC3F2世代中筛选出背景回复率大于理论值93.75%的12个单株,其中6株回复率≥96.92%,田间接种发病率均低于40%。筛选出的6个BC3F3家系中除4JZ574-01外在主要农艺性状上与轮回亲本无显著差异。Five molecular markers closely linked to major resistance loci of head smut in bin2.09 area,were used in this study including 3 STS markers and 2 dCAPS markers.The resistant near-isogenic line 1JD006,which was developed from the susceptible line Huangzao4 and the resistant line Mo17 carrying the major resistance QTL,was used as the resistant donor to improve the susceptible line Chang7-2 combing the marker assistant selection and backcross breeding method.The results showed that 3 M1-25 was the optimal molecular marker with the highest selection efficiency,and next was marker MZA6393.Six plants with high background recovery rates(>87.5%)in BC2F2 population were selected.Twelve plants were selected from BC3F2 population with the recovery rates above96.92%.The field incidence of them were below 40%.Except line 4JZ574-01,the main agronomic traits of the other lines in BC3F3 population were similar to Chang7-2.

关 键 词：玉米 丝黑穗病抗性 分子标记辅助选择 昌7-2 

分 类 号：S513.035.3[农业科学—作物学]