球孢白僵菌生物学性状与对玉米螟毒力相关性分析  

Analysis of the Correlation between the Biological Characteristics of Beauveria bassiana and Its Virulence Against Corn Borer

作　　者：李乐 路杨[1] 赵宇[1] 李启云[1] 徐文静[1] 张正坤[1] 毛刚[1] LI Le;LU Yang;ZHAO Yu;LI Qi-yun;XU Wen-jing;ZHANG Zheng-kun;MAO Gang(Jilin Academy of Agricultural Sciences,Key laboratory of Integrated Pest Management on Crops in Northeast Ministry of Agriculture,Jilin Key Laboratory of Agricultural Microbiology,Changchun 130033;College of Life Sciences,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林省农业科学院/农业部东北作物有害生物综合治理重点实验室/吉林省农业微生物重点实验室,长春130033 [2]吉林农业大学植物保护学院,长春130118

出　　处：《玉米科学》2021年第1期162-169,共8页Journal of Maize Sciences

基　　金：吉林省农业科技创新工程(CXGC2017ZY016);国家重点研发计划项目(2017YFD02006,2017YFD021200)。

摘　　要：球孢白僵菌的生物学性状与对靶标害虫的毒力具有显著相关性,对球孢白僵菌高毒力菌株的初步筛选具有重要意义。鉴定随机分离自亚洲玉米螟幼虫僵虫的10个球孢白僵菌菌株,对其进行产孢量、孢子萌发率、菌落生长速度以及对亚洲玉米螟幼虫的毒力测定。结果表明,不同菌株的生物学特性和毒力均呈现显著差异。在此基础上,分别进行不同菌株生物学性状与毒力的相关性分析。结果表明,球孢白僵菌菌落生长速度与其毒力呈正相关,孢子萌发率与其毒力无相关性。因此,可以将白僵菌菌落生长速度作为白僵菌高毒力菌株筛选的初步评价指标。The biological characteristics of Beauveria bassiana have a significant correlation with its virulence against the target pests,which is of great significance for the preliminary screening of fungal strains with high virulence.Ten isolates of B.bassiana,randomly isolated and identified from a muscardine cadaver of corn borer larva collected from maize field.The sporulation quantity,conidiophores germination rate,colony growth rate and the virulence of the isolates against corn borer third instar larvae were measured,respectively,all of them displayed significant differences among different isolates.On this basis,the correlation between biological characteristics and virulence of all isolates was analyzed.The results showed that for all fungal isolates,the colony growth rate was positively correlated with virulence,the conidiophores germination rate showed no correlation with the virulence.Therefore,the colony growth rate can be used as a preliminary evaluation index for high virulence strains screening of B.bassiana.

关 键 词：球孢白僵菌 产孢量 孢子萌发率 菌落生长速度 毒力 评价指标 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]