低温乙醇法在猪血浆白蛋白提取中的适用性研究  

Research on the Applicability of Low Temperature Ethanol Method in the Extraction of Porcine Plasma Albumin

作　　者：龚慧芳 张淇美 唐青海[1] 阳希 杨海[1] GONG Hui-fang;ZHANG Qi-mei;TANG Qing-hai(Key Laboratory of Conservation and Utilization of Biological Resources in Nanyue Mountain Area,Hengyang Normal University,Hengyang,Hunan 421008)

机构地区：[1]衡阳师范学院南岳山区生物资源保护与利用湖南省重点实验室,湖南衡阳421008 [2]南岳生物制药有限公司,湖南省血液制品工程技术研究中心,湖南衡阳421007

出　　处：《安徽农业科学》2021年第5期172-174,206,共4页Journal of Anhui Agricultural Sciences

基　　金：湖南省自然科学基金-衡阳联合基金项目(2018JJ4023);国家级大学生创新创业训练计划项目(CX1801);衡阳市产学研结合专项(2019)。

摘　　要：[目的]探索猪血浆白蛋白的高效提取方法。[方法]对低温乙醇法在猪血浆白蛋白提取中的适用性进行了研究,设定猪血浆白蛋白提取步骤,并对离心速度、反应时间、pH、适用温度4个关键参数进行优化,对目标蛋白进行定性定量分析。[结果]低温乙醇法适合提取猪血浆白蛋白,最佳提取条件为离心速度8000 r/min,反应时间90 min,pH 4.7,温度-8℃;采用改良后的低温乙醇法,1 L猪血浆可提取白蛋白29.3 g,产品纯度为97.3%,提取时长约10 h。[结论]改良后的低温乙醇法是一种高效提取猪血浆白蛋白的可靠方法。[Objective]To explore an efficient method to extract porcine plasma albumin.[Method]The applicability of low temperature ethanol method in the extraction of porcine plasma albumin was studied,and the four key parameters,centrifugal speed,reaction time,pH and suitable temperature,were optimized;the target protein was analyzed qualitatively and quantitatively.[Result]The results showed that low temperature ethanol method was suitable for the extraction of porcine plasma albumin.The optimum centrifugation speed was 8000 r/min,the reaction time was 90 min,the pH was 4.7,and the temperature was-8℃;the improved low temperature ethanol method could extract 29.3 g albumin from 1 L pig plasma,the purity of the product was 97.3%,and the extraction time was about 10 h.[Conclusion]The improved low temperature ethanol method is a reliable and efficient method for the extraction of porcine plasma albumin.

关 键 词：猪血浆 白蛋白 提取 低温乙醇法 优化 

分 类 号：TS251.93[轻工技术与工程—农产品加工及贮藏工程]