HPLC法同时测定牛黄上清胶囊中8种成分  

Simultaneous Determination of Eight Constituents in NiuHuang ShangQing Capsules by HPLC

作　　者：承晨 徐晶星 CHENG Chen;XU Jing-xing(Changzhou Institute for Food,Drug and Fiber Control,Changzhou 213000,China)

机构地区：[1]常州市食品药品纤维质量监督检验中心,江苏常州213000

出　　处：《海峡药学》2021年第2期66-70,共5页Strait Pharmaceutical Journal

摘　　要：目的建立HPLC法同时测定牛黄上清胶囊中栀子苷、芍药苷、连翘酯苷A、连翘酯苷B、木犀草苷、黄芩苷、盐酸小檗碱、大黄素的含量。方法采用Waters公司的Xbridge C_(18)柱(4.6×250 mm,5μm);流动相为0.1%磷酸-乙腈,梯度洗脱;体积流量1.0 mL·min^(-1);检测波长分别为栀子苷240 nm、芍药苷232 nm、连翘酯苷A与连翘酯苷B 328 nm、木犀草苷与盐酸小檗碱348 nm、黄芩苷277 nm、大黄素288 nm;柱温30℃。结果栀子苷、芍药苷、连翘酯苷B、连翘酯苷A、木犀草苷、黄芩苷、盐酸小檗碱、大黄素的线性范围分别为4.444~444.4μg·mL^(-1)、0.1264~12.64μg·mL^(-1)、1.680~168.0μg·mL^(-1)、1.662~166.2μg·mL^(-1)、0.1629~16.29μg·mL^(-1)、3.926~392.6μg·mL^(-1)、3.624~326.4μg·mL^(-1)和4.157~415.7μg·mL^(-1)(r≧0.9995)。8种成分的平均回收率在92.6%~100.9%之间。结论该方法简便、准确、灵敏、重复性好,可用于牛黄上清胶囊8种成分的含量。OBJECTIVE To establish an HPLC method for simultaneously determination the contents of Geniposide,Paeoniflorin,Forsythoside A,Forsythoside B,Luteolin-7-glucoside,Baicalin,Berberine Hydrochloride and Emodin in Niuhuang Shangqing Capsules.METHODS The analysis was conducted on the Waters′column Xbridge(4.6×250 mm,5μm),which maintained at 30℃column temperature,using 0.1%(v/v)phosphoric acid-acetonitrile in a gradient elution mode as the mobile phase while the flow rate was 1.0 mL·min^(-1).The detection wavelength were set at 240 nm for Geniposide,232 nm for Paeoniflorin,328 nm for Forsythoside A and Forsythoside B,348 nm for Luteolin-7-glucoside and Berberine Hydrochloride,277 nm for Baicalin,and 288 nm for Emodin.RESULTS The correlation coefficients of the equation of linear regression were above 0.9995.The average recoveries between 92.6%-100.9%.CONCLUSION The method is reliable,simple and convenient,which can be used for quality control of Niuhuang Shangqing Capsules.

关 键 词：牛黄上清胶囊 栀子苷 芍药苷 连翘酯苷A 连翘酯苷B 木犀草苷 黄芩苷 盐酸小檗碱 大黄素 高效液相色谱 

分 类 号：R927[医药卫生—药学]