双液相萃取棉仁粉工艺中甲醇相棉子糖的精制  

Purification of Raffionse from Methanol Phase Separated by Two-Phase Solvent Extraction of Cottoseeds

作　　者：顾恒[1,2] 朱庆莉[1] 云志[2] 兰宏兵[3] Gu Heng;Zhu Qingli;Yun Zhi;Lan Hongbing(Jinling Institute of Technology,Nanjing 211169;Nanjing Tech University,Nanjing 211816;College of material and Chemical Engineering,Zhengzhou 450001)

机构地区：[1]金陵科技学院,南京211169 [2]南京工业大学,南京211816 [3]郑州轻工业大学,郑州450001

出　　处：《中国粮油学报》2021年第2期132-137,共6页Journal of the Chinese Cereals and Oils Association

基　　金：金陵科技学院博士科研启动基金(jit-b-201634);金陵科技学院孵化项目基金(jit-fhxm-201922)。

摘　　要：以双液相溶剂(石油醚+甲醇混合)浸取棉仁粉工艺中甲醇相为原料,在预处理的基础上,依次采用硅藻土与活性炭混合物脱色,超滤膜脱蛋白,溶剂丙酮-乙醇-水(体积比2∶1∶0.5)重结晶的方法精制棉子糖,并以棉子糖收率和脱色率(或纯度)为指标,优化纯化工艺。结果表明最佳工艺条件为:脱色:在50℃下,采用3%(混合物与棉子糖溶液质量比)的硅藻土与活性炭混合物对棉子糖粗品进行2 h脱色;超滤:棉子糖溶液pH值为8,温度40℃,操作压力0.016 MPa;重结晶:在料液比1∶14下,冷冻重结晶24 h。最终得到棉子糖的纯度为94.5%,回收率为63.1%。The methanol phase from Two-phase solvent(methanol and petroleum ether)extraction was used as the raw meterial.Based on the pretreatment,it was subjected in the following order:bleaching with the mixture of diatomaceous earth and activated characoal,ultrafiltrating with a hollow-fiber membrane,and raffinose is refined by recrystallization using the mixture of volume ratio of t acetone-ethanol-water(2∶1∶0.5).It was found that the optimum conditions were as followed:decolorization:the amount of mixture of 3%,the temperature of 50℃,and the time of 2 h;ultrafiltraction:pH=8,raffinose solution under 0.016 MPa pressure,at 40℃;solvent recrystallization:the ratio of solid to liquid of 1∶14,the recrystallization time of 24 h.Under these conditions,the final purity of raffinose crystal could be up to 94.5%,the recovery rate was 63.1%.

关 键 词：棉子糖 提纯 脱色 超滤 重结晶 

分 类 号：TS224.4[轻工技术与工程—粮食、油脂及植物蛋白工程]