高产不饱和油脂海鞘真菌桔青霉Asc-2-4的诱变选育  

Mutation Breeding of Unsaturated Lipid High-Yielding Penicillium citrinum Asc-2-4 from Ascidian

作　　者：罗国聪 柴慧子 雷晓凌[1] 聂芳红[1] 罗正东 LUO Guo-cong;CHAI Hui-zi;LEI Xiao-ling;NIE Fang-hong;LUO Zheng-dong(Coll.of Food Sci.&Technol.,Guangdong Ocean Uni.,Zhanjiang 524088;Inst.of Pathogen.Microorg.,Jinan Uni.,Guangzhou 510632)

机构地区：[1]广东海洋大学食品科技学院,广东湛江524088 [2]暨南大学病原微生物研究所,广东广州510632

出　　处：《微生物学杂志》2020年第6期7-12,共6页Journal of Microbiology

基　　金：广东海洋大学国家级大学生创新项目(201710566009);湛江市科学技术局项目(2016A02020)。

摘　　要：诱变育种是获得高产菌株,实现微生物工业化生产油脂的重要措施。以前期获得的高产不饱和油脂菌株桔青霉(Penicillium citrinum)Asc-2-4为出发菌株,利用丙二酸建立快速筛选高产不饱和脂肪酸突变菌的方法,通过紫外线-氯化锂复合诱变得到1株高产油脂突变菌Asc-2-4-1,油脂含量比出发菌株提高了92.98%。经过初步的培养基无机盐优化,其油脂得率和不饱和脂肪酸产量达到了7.10 g/L和3.84 g/L,与Asc-2-4相比,分别提高了84.42%和77.78%。结果表明,通过复合诱变选育技术可选育出高产突变菌株,选育的Asc-2-4-1可望作为产油微生物被开发利用。Mutation breeding is an important measure to acquire high-yielding strains for microbial industrialization of oil production.In this study a previously obtained strain Asc-2-4 with unsaturated lipid-high yielding was used as starting strain,and adopted a rapid screening method for unsaturated lipid mutant strain established by malonic acid,and mutated with UV-LiCl compound mutation,an oil high-yielding mutant strain Asc-2-4-1 was obtained.The oil content increased by 92.98%as compared with the starting strain.After initial optimization with metal ion cultivation the gain rate of oil was as high as 7.10 g/L and unsaturated fatty acid 3.84 g/L,as compared with Asc-2-4,they increased respectively by 84.42%and 77.78%.The results showed that adopting compound mutation breeding technology could breed high-yielding mutant strain and the bred strain Asc-2-4-1 is a hopeful oil-producing microbe to be developed and utilized.

关 键 词：海鞘真菌 诱变育种 产油脂真菌 

分 类 号：Q939.97[生物学—微生物学]