一株产酸克雷伯氏菌发酵培养基优化  

Optimization of Fermentation Medium for Acid-Producing Klebsiella oxytoca

作　　者：杨丹丹 张心青 杨传伦 马娜娜 李佳明 YANG Dan-dan;ZHANG Xin-qing;YANG Chuan-lun;MA Na-na;LI Jia-ming(Yellow River Delta Chambroad Chem.Indust.Res.Inst.Co.,Ltd.Binzhou 256500;Shandong Chambroad Environ′l Protect.Material Co.Ltd.,Binzhou 256500)

机构地区：[1]黄河三角洲京博化工研究院有限公司,山东滨州256500 [2]山东京博环保材料有限公司,山东滨州256500

出　　处：《微生物学杂志》2020年第6期75-83,共9页Journal of Microbiology

基　　金：山东省重点研发计划项目(2016ZDJSD7A18)。

摘　　要：为提高产酸克雷伯氏菌(Klebsiella oxytoca)的发酵水平,通过单因素优化,研究培养条件、碳源、氮源、无机盐对产酸克雷伯氏菌活菌量的影响,利用响应面分析法对影响产酸克雷伯氏菌活菌量的关键因子进行优化,30 L发酵罐进行扩大培养。得出最佳配方(质量分数):黄豆饼粉2.19%,玉米粉1.0%,蔗糖1.10%,硫酸铵0.06%,玉米浆干粉0.5%,蛋白胨0.05%,硫酸镁0.04%,磷酸二氢钾0.02%,氯化钠0.080%,硫酸锰0.03%,pH 7.0~7.4。活菌量稳定在2.0×1010 cfu/mL以上,能够满足生产要求,为产酸克雷伯氏菌作为微生物菌肥的应用提供参考。In order to improve the fermentation level of acid-producing Klebsiella oxytoca.Single factor optimization was firstly carried out to study the influence of culture conditions,carbon source,nitrogen source and inorganic salt on the amount of viable acid-producing K.oxytoca;then the key factors affecting the activity were optimized by response surface analysis;finally,30 L fermentor for expanded culture.And obtained the optimized medium formula:2.19%soybean cake powder,1.0%corn meal,1.10%sucrose,0.06%(NH)2SO 4,0.5%corn syrup pulp dry powder,0.05%peptone,0.04%MgSO 4,0.02%KH 2PO 4,0.080%NaCl,0.03%MnSO 4,at pH 7.0-7.4.The amount of viable bacteria can be stable above 2.0×1010 cfu/mL,and can meet the production requirements and provide technical support for the application of acid-producing K.oxytoca microbial fertilizer.

关 键 词：产酸克雷伯氏菌 微生物菌肥 单因素优化 活菌量 响应面优化 发酵罐 

分 类 号：Q939.96[生物学—微生物学]