金线莲多糖含量的测定及组织化学定位  

Content Determination and Histochemical Localization of Polysaccharide from Anoectochilus roxburghii

作　　者：张碧玉[1] 林艺华[2] 邹毅辉[2] 林美珍[2] ZHANG Bi-yu;LIN Yi-hua;ZOU Yi-hui;LIN Mei-zhen(Pharmacy of Traditional Medicine,Zhangzhou Affiliated Hospital of Fujian Medical University,Zhangzhou 363000,China;Department of Pharmacy,Zhangzhou Health Vocational College,Zhangzhou 363000,China)

机构地区：[1]漳州市医院中药房,福建漳州363000 [2]漳州卫生职业学院药学系,福建漳州363000

出　　处：《海峡药学》2021年第1期49-51,M0004,共4页Strait Pharmaceutical Journal

基　　金：漳州市自然科学基金项目(ZZ2020J07);漳州卫生职业学院科技创新团队培育计划(Kjcx-3)。

摘　　要：目的测定金线莲不同器官的多糖含量及组织定位。方法微波-超声协同萃取金线莲根、茎、叶中的多糖,并用苯酚-硫酸法测定其含量,用植物组织化学方法定位多糖的贮存部位。结果金线莲茎的多糖含量为4.47%,根为2.12%,叶为0.64%;金线莲中的多糖贮存于根茎的皮层薄壁细胞。结论金线莲多糖贮存于根茎的薄壁细胞,且茎的含量高于根,叶的多糖含量极少;本研究为金线莲高效利用奠定基础。OBJECTIVE To determine the polysaccharide content and locate polysaccharide from different organs of Anoectochilus roxburghii Lind1.METHODS The polysaccharide was extracted from the root,stem and leaf of A.roxburghii by microwave-ultrasonic method and its content was determined by phenol-sulfuric acid method.Phytohistochemistry was used to locate the storage sites of polysaccharides.RESULTS The content of polysaccharide in stem,root,leaf orderly was 4.47%,2.12%,0.64%.The polysaccharide is stored in the cortex parenchyma cells of the rhizome.CONCLUSION The polysaccharide is stored in the parenchyma cells of rhizome,and the polysaccharide content from stem was higher than that from root,while the polysaccharide content from leaf was very little.This study laid a foundation for the efficient utilization of A.roxburghii.

关 键 词：金线莲 多糖 含量测定 组织定位 

分 类 号：R284[医药卫生—中药学]