HPLC法同时测定畅脉乐Ⅰ号胶囊中葛根素、芍药苷和丹酚酸B的含量  

Simultaneous Determination of Puerarin Paeoniflorin and Salvianolic Acid B in ChangMaiLeⅠCapsules by HPLC

作　　者：陈丽艳 CHEN Li-yan(Fuzhou Institute for Food and Drug Control,Fuzhou 350007,China)

机构地区：[1]福州市食品药品检验所,福建福州350007

出　　处：《海峡药学》2021年第1期77-80,共4页Strait Pharmaceutical Journal

摘　　要：目的建立HPL法同时测定畅脉乐Ι号胶囊中葛根素、芍药苷和丹酚酸B三种成分的含量。方法选择Waters XBridge C_(18) ODS (250 mm×4.6 mm,5μm)色谱柱,以乙腈(CH_3CN)-甲醇(CH_3OH)-0.05%磷酸(H_3PO_4)为流动相,梯度洗脱,流速1.0 mL·min^(-1),检测波长230 nm,柱温25℃,进样量:10μL。结果葛根素、芍药苷和丹酚酸B的线性范围分别为6.272^156.80μg·mL^(-1)(r=0.9998),2.56^64.10μg·mL^(-1)(r=0.9997),2.0588^62.72μg·mL^(-1)(r=0.9998);平均回收率(n=9)分别为97.53%、97.50%和97.62%,RSD分别为1.15%、1.31%和1.48%。结论本法操作简便,结果准确可靠,可更好用于畅脉乐Ι号胶囊中葛根素、芍药苷和丹酚酸B的含量测定。OBJECTIVE To develop a HPLC method for simultaneous determination of three ingredients of puerarin,paeoniflorin and salvianolic acid B in ChangMaiLeⅠcapsules.METHODS Chromatographic column:Waters XBridge C 18 ODS(250 mm×4.6 mm,5μm);Mobile phase:acetonitrile and methanol and 0.05%phosphoric acid,gradientelution;Flow rate:1 mL·min-1;Wavelengh:230 nm;Column temperature:25℃.RESULTS The linear ranges of puerarin,paeoniflorin and salvianolic acid B were 6.272-156.8μg·mL-1(r=0.9998),2.564-64.10μg·mL-1(r=0.9997),2.0588-62.72μg·mL-1(r=0.9998),respectively.The average recoveries of puerarin,paeoniflorin and salvianolic acid B were 97.53%with RSD 1.15%,97.50%with RSD 1.31%and 97.62%with RSD 1.48%,respectively.CONCLUSION The method is simple,accurate and can be used for the simultaneous determination of puerarin、paeoniflorin and salvianolic acid B in ChangMaiLeⅠcapsules.

关 键 词：畅脉乐Ⅰ号胶囊 葛根素 芍药苷和丹酚酸B HPLC 含量测定 

分 类 号：R927[医药卫生—药学]