mSdr9c7基因在C57BL/6J小鼠不同组织中表达水平的研究  

Study on the Expression Level of mSdr9c7 Gene in Different Tissues of C57BL/6J Mice

作　　者：李银玲 周洁[1,2] 周晶 陈俏媛 曾志棚 林万华 LI Yinling;ZHOU Jie;ZHOU Jing;CHEN Qiaoyuan;ZENG Zhipeng;LIN Wanhua(Guangxi Universities Key Laboratory of Stem Cell and Biopharmaceutical Technology(Guangxi Normal University),Guilin Guangxi 541004,China;College of Life Sciences,Guangxi Normal University,Guilin Guangxi 541006,China)

机构地区：[1]广西高校干细胞与医药生物技术重点实验室(广西师范大学),广西桂林541004 [2]广西师范大学生命科学学院,广西桂林541006

出　　处：《广西师范大学学报（自然科学版）》2021年第1期148-155,共8页Journal of Guangxi Normal University:Natural Science Edition

基　　金：国家自然科学基金(31560248);广西自然科学基金(2016GXNSFAA380176)。

摘　　要：为探讨mSdr9c7基因在C57BL/6J雄性成年小鼠各组织中的表达情况,进一步研究mSdr9c7基因的功能,本文筛选适于实时荧光定量PCR法检测mSdr9c7基因表达水平的引物(正向引物序列为F:GGCCTGGACGTCAAGTTTCT,反向引物序列为R:TTCTAGAGGCCCCAGCAGAT),以5只18周龄C57BL/6J雄性小鼠为供试动物,采集其皮肤、肝脏、睾丸等16种组织,并从外周血中分离红细胞、白细胞,采用实时荧光定量PCR法检测mSdr9c7基因16种小鼠组织或细胞中mRNA的表达情况。以每只小鼠相应肝脏组织的表达量作为参照并进行统计分析,结果表明:mSdr9c7 mRNA在皮肤、肝脏、睾丸、骨髓组织为高水平表达,分别是肝脏组织的1.069±0.0989、1.010±0.0508、0.987±0.0810、0.822±0.0838倍;在血管、白细胞、大脑、血液、肾脏、肺、十二指肠、胃、外周红细胞、脾脏等组织或细胞中为中等水平表达,分别是肝脏组织的0.697±0.0530、0.628±0.0570、0.577±0.0996、0.522±0.0622、0.538±0.0535、0.455±0.0715、0.439±0.0869、0.375±0.1019、0.391±0.0320、0.377±0.0520倍;而在脂肪、肌肉、小脑、心脏为低水平表达,分别是肝脏组织的0.189±0.0256、0.129±0.0343、0.118±0.0639、0.052±0.0140倍。The expression level of mSdr9c7 gene in the tissues of C57BL/6J male adult mice was detected for further research on its function.The suitable forward primer sequence as GCCTGACGTCAAGTTCT and the backward primer sequence as TTCTAGAGGCCCCAGCAGAT for real-time fluorescence quantitative PCR were screened to detect the expression level of mSdr9c7 gene.Five 18-week-old C57BL/6J male mice were used as experimental animals.16 kinds of tissues or cells such as skin,liver,testis,bone marrow,blood vessels,cerebellum,cerebellum,kidney,blood,lung,duodenum,spleen,stomach,fat,muscle and heart were collected,and red blood cells and lymphocyte were isolated from peripheral blood.The real-time fluorescent quantitative PCR method was used to detect the expression of mSdr9c7 gene mRNA in the above-mentioned mouse tissues or cells.The gene expression the corresponding liver tissues of each mouse was used as a reference and statistically analyzed.The results showed that mSdr9c7 mRNA was highly expressed in skin,liver,testis and bone marrow,which was 1.069±0.0989,1.010±0.0508,0.987±0.0810 and 0.822±0.0838 times as that in liver tissue respectively.It expressed at a moderate level in blood vessels,lymphocyte,brain,blood,kidney,lung,duodenum,stomach,red blood cells and spleen,and they were 0.697±0.0530,0.628±0.0570,0.577±0.0996,0.522±0.0622,0.538±0.0535,0.455±0.0715,0.439±0.0869,0.375±0.1019,0.391±0.0320,0.377±0.0520 times as that in liver tissue respectively.Its expression was at a low level in fat,muscle,cerebellum and heart,which was 0.189±0.0256,0.129±0.0343,0.118±0.0639 and 0.052±0.0140 times as that in liver tissue respectively.

关 键 词：mSdr9c7基因 C57BL/6J小鼠 组织表达 

分 类 号：Q28[生物学—细胞生物学]