松郁安神方对PCPA致失眠大鼠睡眠时相的影响  

Effect of Songyu Anshen Formula on Sleep Phase in Insomnia Rats Induced by Para-chlorophenylalanine(PCPA)

作　　者：曾雪爱 周春权 吴华嵩 黄俊山 ZENG Xueai;ZHOU Chunquan;WU Huasong;HUANG Junshan(Fujian Academy of Chinese Medicine Sciences,Fuzhou 350003,China)

机构地区：[1]福建省中医药科学院,福州350003

出　　处：《世界睡眠医学杂志》2021年第1期7-10,共4页World Journal of Sleep Medicine

基　　金：国家自然科学基金项目(81704072);福建省科技厅公益类科研院所专项重点项目(2017R1035-2);福建省中医药科研项目(2017FJZYJC101)。

摘　　要：目的:观察松郁安神方对失眠大鼠睡眠时相的影响。方法:采用腹腔注射对氯苯丙氨酸(Para-chlorophenylalanine,PCPA)建立失眠大鼠模型,用松郁安神方进行干预,通过动物睡眠生物解析系统,记录脑电(Electroencephalogram,EEG)和肌电(Electromyogram,EMG),分析睡眠觉醒时间、非快速眼动睡眠(Non-rapid Eyemovement Sleep,NREM)时间和快速眼动睡眠(Rapid Eyemovement Sleep,REM)时间,观察大鼠睡眠时相变化。结果:PCPA可明显减少大鼠白天NREM、REM睡眠时间,增加觉醒时间,给予松郁安神方后,失眠大鼠白天时段NREM、REM睡眠时间增加,觉醒时间减少,与PCPA模型组比较,差异有统计学意义(P<0.05或P<0.01)。结论:松郁安神方能改善PCPA致失眠大鼠的睡眠,其主要通过增加白天的NREM和REM睡眠时间改善睡眠。Objective:To observe the effect of Songyu Anshen formula on sleep phase in insomnia rats.Methods:Para-chlorophenylalanine(PCPA)was injected intraperitoneally to establish the model of insomnia rats.The rats were administrated intragastrically with Songyu Anshen formula.The changes of sleep phases in rats were observed by recording electroencephalogram(EEG)and electromyogram(EMG),and the amount of waking,non-rapid eyemovement(NREM)sleep and rapid eyemovement(REM)sleep were analyzed.Results:PCPA can significantly reduce NREM and REM sleep duration,and increase the wakefulness time in daytime.Songyu Anshen formula can significantly increase NREM and REM sleep duration,and reduce the wakefulness time of insomnia rats induced by PCPA during daytime(P<0.05,P<0.01,P<0.001).Conclusion:Songyu Anshen formula can improve sleep quality of insomnia rats,mainly by increasing NREM and REM sleep duration in the daytime.

关 键 词：失眠 松郁安神方 睡眠时相 

分 类 号：R256.23[医药卫生—中医内科学]