miR-29b抑制LPS诱导的人支气管上皮细胞系16HBE的凋亡  

miR-29b inhibits LPS-induced apoptosis in human bronchial epithelial cell line 16HBE

作　　者：袁东 蒋瑶娜 李亚清[3] YUAN Dong;JIANG Yao-na;LI Ya-qing(Graduate School of Bengbu Medical College, Bengbu 233030;the Second Clinical Medical College of Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053;Department of Respiratory Medicine,Zhejiang Provincial People's Hospital, Hangzhou 310014, China)

机构地区：[1]蚌埠医学院研究生院,安徽蚌埠233030 [2]浙江中医药大学第二临床医学院,浙江杭州310053 [3]浙江省人民医院呼吸内科,浙江杭州310014

出　　处：《基础医学与临床》2021年第3期333-339,共7页Basic and Clinical Medicine

基　　金：国家自然科学基金(8187010018)。

摘　　要：目的研究miR-29b对脂多糖(LPS)诱导的人支气管上皮细胞系16HBE凋亡的影响。方法将16HBE细胞分为对照组、LPS组(含50μg/mL LPS的细胞培养液培养)、转染mimics control组和转染miR-29b mimics组。MTT法检测增殖;流式细胞测量术检测凋亡;Western blot检测c-caspase-3、c-caspase-12、糖调节蛋白78(GRP78)和CCAAT/增强子结合蛋白同源蛋白(CHOP)蛋白表达。在16HBE细胞中共转染miR-29b mimics、pcDNA-CHOP,同样利用上述方法检测增殖、凋亡。结果与对照组比较,LPS组细胞增殖活性降低、凋亡率升高(P<0.05),细胞中c-caspase-3、c-caspase-12、GRP78和CHOP蛋白表达水平升高(P<0.05)。与转染mimics control组比较,转染miR-29b mimics组细胞增殖活性升高、凋亡率降低(P<0.05),细胞中c-caspase-3、c-caspase-12、GRP78和CHOP蛋白表达水平降低(P<0.05)。pcDNA-CHOP可以逆转miR-29b mimics对LPS条件下支气管上皮细胞增殖和凋亡的影响。结论miR-29b抑制LPS诱导的16HBE细胞的凋亡,其作用机制与抑制内质网应激有关。Objective To study the effect of miR-29b on lipopolysaccharide(LPS)-induced apoptosis of human bronchial epithelial cell line 16HBE.Methods 16HBE cells were divided into control group,LPS group(LPS treatment),transfection mimics control group,transfection miR-29b mimics group.MTT assay for proliferation,flow cytometry was used for apoptosis;Western blot was used for checking the expression of cleaved cysteinyl aspartate specific proteins 3(c-caspase-3),cleaved cysteinyl aspartate specific proteinase 12(c-caspase-12),glucose-regulated protein 78(GRP78)and CCAAT/enhancer-binding protein C/EBP(CHOP)protein.Human bronchial epithelial cells 16HBE were co-transfected with miR-29b mimics and pcDNA-CHOP,the proliferation and apoptosis were also determined using the above method.Results Compared with the control group,the cell proliferation-activity and apoptosis rate of the LPS group decreased,and the protein expression level of c-caspase-3,c-caspase-12,GRP78,and CHOP all increased.Compared with the LPS+NC group,the LPS+miR-29b group showed an increased cell proliferation,decreased apoptosis and decreased expression of c-caspase-3,c-caspase-12,GRP78,and CHOP protein.pcDNA-CHOP reversed the effects of miR-29b mimics on the proliferation and apoptosis of bronchial epithelial cells under LPS conditions.Conclusions miR-29b inhibits 16HBE apoptosis induced by LPS;the mechanism might be related to the inhibition of endoplasmic reticulum stress.

关 键 词：miR-29b 支气管上皮细胞 脂多糖 凋亡 

分 类 号：R562.2[医药卫生—呼吸系统]