甲基绿吸收光谱法同时测定饮料中的酒石黄和日落黄  

Simultaneous determination of tartrazine and sunset yellow in beverages by methyl green absorption spectrometry

作　　者：江虹[1] 庞向东[1] JIANG Hong;PANG Xiangdong(College of Chemistry and Chemical Engineering,Yangtze Normal University,Chongqing 408100,China)

机构地区：[1]长江师范学院化学化工学院,重庆408100

出　　处：《食品与发酵工业》2021年第5期238-243,共6页Food and Fermentation Industries

基　　金：重庆市教委科技基金资助项目(S201910647003);长江师范学院科技基金资助项目(2018CXX111)。

摘　　要：研究建立了一种简便、快速、能同时测定饮料中共存着色剂——酒石黄和日落黄的吸收光谱新方法。在pH 9.43 Tris-HCl溶液中,甲基绿与酒石黄和日落黄发生显色反应,生成离子缔合物,在400~750 nm范围内,酒石黄体系在574 nm波长处产生1个能进行定量分析的特征负吸收峰,共存色素日落黄不干扰测定,酒石黄的质量浓度在0.04~16.0 mg/L范围内与缔合物的吸光度绝对值(A)有很好的线性关系,表观摩尔吸光系数为4.90×10^(4) L/(mol·cm),检出限为0.033 mg/L,饮料的定量限为1.80 mg/kg;日落黄体系在488 nm波长处产生1个能用于定量分析的特征正吸收峰,共存色素酒石黄不干扰测定,日落黄的质量浓度在0.04~13.6 mg/L范围内与缔合物的吸光度有很好的线性关系,表观摩尔吸光系数为4.19×10^(4) L/(mol·cm),检出限为0.028 mg/L,饮料的定量限为1.51 mg/kg。该方法简便、快速,通过控制溶液酸度便可在不经分离的情况下同时测定同一饮料样品中共存色素——酒石黄和日落黄的含量。A simple,rapid and new absorption spectra method for the simultaneous determination of the coexisting colorants—tartrazine and sunset yellow in beverages was developed.In the pH 9.43 Tris-HCl solution,methyl green reacted with tartrazine and sunset yellow to form ionic association complexes.Within the range of 400-750 nm,the tartrazine system produced a characteristic negative absorption peak that can be quantitatively analyzed at the wavelength of 574 nm,and the coexistence pigment sunset yellow did not interfere with the determination of tartrazine.The mass concentration of tartrazine in the range of 0.04-16.0 mg/L had a good linear relationship with the absorbance of the association complex,the apparent molar absorbance coefficient was 4.90×10^(4) L/(mol·cm)with the detection limit of 0.033 mg/L,and the quantitative limit of beverage was 1.80 mg/kg.The sunset yellow system produced a characteristic positive absorption peak that can be quantitatively analyzed at the wavelength of 488 nm,and the coexistence pigment tartrazine did not interfere with the determination of sunset yellow.Moreover,the mass concentration of sunset yellow in the range of 0.04-13.6 mg/L had a good linear relationship with the absorbance of the associative complex,the apparent molar absorbance coefficient was 4.19×10^(4) L/(mol·cm)with the detection limit of 0.028 mg/L,and the quantitative limit of beverage was 1.51 mg/kg.The method was simple,rapid,and it could simultaneously determine the content of coexisting pigments—tartrazine and sunset yellow in the same beverage sample without separation by controlling the acidity of the solution,with the satisfactory results.

关 键 词：甲基绿 酒石黄 日落黄 酸度控制 吸收光谱法 

分 类 号：TS27[轻工技术与工程—农产品加工及贮藏工程] O657.3[轻工技术与工程—食品科学与工程]