Rapid generation of ACE2 humanized inbred mouse model for COVID-19 with tetraploid complementation  

作　　者：Feng-Liang Liu Kaixin Wu Jiaoyang Sun Zilei Duan Xiongzhi Quan Junqi Kuang Shilong Chu Wei Pang Han Gao Ling Xu Ying-Chang Li Hai-Lin Zhang Xue-Hui ang Rong-Hua Luo Xiao-Li Feng Hans RScholer Xinwen Chen Duanqing Pei Guangming Wu Yong-Tang Zheng Jiekai Chen 

机构地区：[1]Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences/Key Laboratory of Bioactive Peptides of Yunnan Province,KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases,Kunming Institute of Zoology,Chinese Academy of Sciences,China [2]Center for Cell Fate and Lineage(CCLA),Bioland Laboratory(Guangzhou Regenerative Medicine and Health Guangdong Laboratory),China [3]CAS Key Laboratory of Regenerative Biology,Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,China [4]University of the Chinese Academy of Sciences,China [5]Kunming National High-level Biosafety Research Center for Non-human Primates,Center for Biosafety Mega-Science,Kunming Institute of Zoology Chinese Academic of Sciences,China [6]Joint School of Life Science,Guangzhou Medical University,China [7]Department of Developmental Biology,School of Basic Medical Sciences,Southern Medical University,China [8]Centra I Laboratory,The Sixth Affiliated Hospital of Guangzhou Medical University,Qingyuan Peoples Hospital,China [9]Department of Cell and Developmental Biology,Max Planck Institute for Molecular Biomedicine,Germany [10]Medical Faculty,University of Munster,Germany

出　　处：《National Science Review》2021年第2期9-11,共3页国家科学评论（英文版）

基　　金：emergency grants for prevention and control of SARS-Co V-2 from the Ministry of Science and Technology(2020YFC0842400);Science and Technology Planning Project of Guangdong Province,China(2020B111108001,2020B1212060052);Key Research&Development Program of Bioland Laboratory(Guangzhou Regenerative Medicine and Health Guangdong Laboratory)(2018GZR110104003);the National Key Research and Development Program of China(2019YFA0110200);the National Natural Science Foundation of China(31970879,32070794,32000503 and U1902210);Science and Technology Planning Project of Guangzhou(202008040005);the special project for COVID-19 of Bioland Laboratory(2020GZR110106006,2020GZR110106007);the Frontier Science Research Program of the CAS(ZDBS-LY-SM007)。

摘　　要：Although monkeys and pigs can be infected with SARS-Co V-2,they generally have a long growth cycle and a large size that is difficult to handle in large quantities.However,the readily available rats and mice are not susceptible to SARSCo V-2 because of differences in ACE2(angiotensin-converting enzyme 2),the receptor mediating cell entry of SARSCo V-2[1].Thus,the key to establishing a mouse model of COVID-19 is to make the mice express human ACE2 protein,and therefore become SARS-Co V-2 susceptible.Recently reported COVID-19 mouse models using a random insertion transgene approach[2,3]or adenoviral approach to express h ACE2[4],lack the tissue-specificity of h ACE2 expression and might not replicate COVID-19 precisely,limiting their applications in certain studies.

关 键 词：ACE2 SPECIFICITY establishing 

分 类 号：Q95-33[生物学—动物学]