Postsynaptic Targeting and Mobility of Membrane Surface-Localized hASIC1a  

作　　者：Xing-Lei Song Di-Shi Liu Min Qiang Qian Li Ming-Gang Liu Wei-Guang Li Xin Qi Nan-Jie Xu Guang Yang Michael Xi Zhu Tian-Le Xu 

机构地区：[1]Center for Brain Science of Shanghai Children’s Medical Center,Shanghai Jiao Tong University School of Medicine,Shanghai 200127,China [2]Department of Anatomy and Physiology,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China [3]Shanghai Research Center for Brain Science and Brain-Inspired Intelligence,Shanghai 201210,China [4]Shanghai Institute for Advanced Immunochemical Studies,ShanghaiTech University,Shanghai 201210,China [5]Department of Integrative Biology and Pharmacology,McGovern Medical School,University of Texas Health Science Center at Houston,Houston,TX 77030,USA

出　　处：《Neuroscience Bulletin》2021年第2期145-165,共21页神经科学通报（英文版）

基　　金：This work was supported by grants from the National Natural Science Foundation of China(81961128024 and 81730095);the Innovative Research Team of High-level Local Universities in Shanghai;the Science and Technology Commission of Shanghai Municipality(18JC1420302);the Shanghai Municipal Science and Technology Major Project(2018SHZDZX05);the US National Institutes of Health(NS114716).

摘　　要：Acid-sensing ion channels(ASICs),the main H^(+)receptors in the central nervous system,sense extracellular pH fluctuations and mediate cation influx.ASIC1a,the major subunit responsible for acid-activated current,is widely expressed in brain neurons,where it plays pivotal roles in diverse functions including synaptic transmission and plasticity.However,the underlying molecular mechanisms for these functions remain mysterious.Using extracellular epitope tagging and a novel antibody recognizing the hASIC1a ectodomain,we examined the membrane targeting and dynamic trafficking of hASIC1a in cultured cortical neurons.Surface hASIC1a was distributed throughout somata and dendrites,clustered in spine heads,and co-localized with postsynaptic markers.By extracellular pHluorin tagging and fluorescence recovery after photobleaching,we detected movement of hASIC1a in synaptic spine heads.Single-particle tracking along with use of the anti-hASIC1a ectodomain antibody revealed long-distance migration and local movement of surface hASIC1a puncta on dendrites.Importantly,enhancing synaptic activity with brain-derived neurotrophic factor accelerated the trafficking and lateral mobility of hASIC1a.With this newly-developed toolbox,our data demonstrate the synaptic location and high dynamics of functionallyrelevant hASIC1a on the surface of excitatory synapses,supporting its involvement in synaptic functions.

关 键 词：ASIC1a Surface labeling Visualization Membrane trafficking Brain-derived neurotrophic factor Synaptic function 

分 类 号：R338[医药卫生—人体生理学]