一个水稻斑马叶突变体的遗传分析和基因定位  

Genetic analysis and gene mapping of a zebra leaf mutant in rice

作　　者：陈海元 朱晓妹 张所兵[1] 张云辉[1] 方先文[1] CHEN Hai-yuan;ZHU Xiao-mei;ZHANG Suo-bing;ZHANG Yun-hui;FANG Xian-wen(Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)

机构地区：[1]江苏省农业科学院种质资源与生物技术研究所,江苏南京210014

出　　处：《江苏农业学报》2021年第1期1-7,共7页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省自然科学基金项目(BK20180309)。

摘　　要：通过甲基磺酸乙酯(EMS)诱变,获得了1个可以稳定遗传的水稻斑马叶突变体zebra2-2。与野生型相比,突变体从苗期开始表现出黄绿相间的斑马叶表型,而且不同叶位叶片的表型存在差异,新叶较老叶的表型更加明显。30℃条件下可恢复为绿-淡黄相间表型。此外,突变体的抽穗期延迟,株高、穗长、每穗粒数和籽粒大小均显著降低。遗传分析结果表明,突变体的突变表型受1对隐性基因控制。图位克隆结果表明,突变体ZEBRA2基因的第3342位碱基由G突变为A,使得编码的氨基酸由甘氨酸突变成天冬氨酸,导致突变体呈现斑马叶表型。A zebra leaf mutant zebra2-2 with stable inheritance was identified through ethyl methanesulfonate(EMS)mutagenesis.Compared with the wild type,the mutant displayed yellow and green zebra leaf phenotype since seeding stage,and leaves at different positions showed variable mutant phenotypes,new leaves showed more significant zebra leaf phenotype than the older leaves.Seedlings at 30℃could recover the yellow and green zebra leaf phenotype.In addition,the heading date of mutant was delayed,and the plant height,panicle length,spikelet number per panicle and kernel size were significantly reduced.Genetic analysis demonstrated that the mutant phenotype was controlled by a single recessive nuclear gene.Map-based cloning results revealed that a single base G to A substitution at the 3342 position from ATG start codon,caused a Gly-to-Asp substitution of ZEBRA2 gene in zebra2-2,which resulting in the zebra leaf phenotype of zebra2-2.

关 键 词：水稻 斑马叶 遗传分析 基因定位 

分 类 号：S511.035.2[农业科学—作物学]