肉芽组织下注射微粒皮浆对大鼠创伤性慢性创面愈合的影响  

Effect of granulation tissue injection of micropar skin pulp on the healing of traumatic chronic wound in rats

作　　者：祁永章[1] 李毅[1] 梁琰 沈丛墨 曹晓婷 Qi Yongzhang;Li Yi;Liang Yan;Shen Congmo;Cao Xiaoting(Department of Burns and Plastic Surgery, Affiliated Hospital of Qinghai University, Xining 810001, China)

机构地区：[1]青海大学附属医院烧伤整形科,西宁810001

出　　处：《中华损伤与修复杂志（电子版）》2020年第6期475-481,共7页Chinese Journal of Injury Repair and Wound Healing(Electronic Edition)

基　　金：青海大学附属医院中青年科研项目(ASRE-2018-YB-05)。

摘　　要：目的研究肉芽组织下注射微粒皮浆对大鼠创伤性慢性创面愈合的作用。方法选取60只SD雄性大鼠,于背部制作大小为3.0 cm×3.0 cm的创面,并将钢丝圈缝于创面内缘。按照随机数字表法将大鼠分为3组,分别为一般创面组、慢性创面组和微粒皮浆组,每组各20只。一般创面组背部造成创面后给予抗感染治疗并常规换药;慢性创面组背部形成创面后给予抗感染治疗、常规换药,并连续7 d肌内注射氢化可的松干预形成慢性创面;微粒皮浆组背部形成创面后给予抗感染治疗、常规换药,连续7 d肌内注射氢化可的松干预形成慢性创面,取大鼠右大腿外侧皮肤制备微粒皮浆注射于肉芽组织下。造模完成次日开始观察创面情况,定为观察第1天。观察第7、14、21、28天各组创面愈合情况,并计算创面愈合率;留取观察第14天的肉芽组织进行苏木精-伊红染色以及CD31免疫组织化学染色,观察苏木精-伊红染色下创面新生毛细血管分布情况及CD31免疫组织化学染色下CD31表达情况与微血管密度。对数据行单因素方差分析和LSD-t检验。结果观察第14天,一般创面组创面明显缩小,慢性创面组皮肤爬伸不明显,微粒皮浆组创面大部分愈合;观察第28天,一般创面组剩余部分残留创面,慢性创面组创面愈合不明显,微粒皮浆组创面基本愈合。观察第14、21、28天,一般创面组愈合率分别为(51.09±0.94)%、(75.43±0.92)%、(86.51±0.57)%,慢性创面组创面愈合率分别为(20.30±0.95)%、(35.59±1.18)%、(45.82±1.35)%,微粒皮浆组创面愈合率分别为(39.00±0.86)%、(64.62±0.15)%、(91.25±0.87)%,比较差异均有统计学意义(F=1993.60、6475.02、9984.47,P值均小于0.05);观察第14天,慢性创面组创面愈合率分别与一般创面组、微粒皮浆组比较,差异均有统计学意义(t=89.90、50.93,P值均小于0.05);观察第21天,慢性创面组创面愈合率分别与一般创面组、微粒皮浆组�Objective To study the effect of granulation tissue injection of micropar skin pulp on the healing of traumatic chronic wound in rats.Methods Sixty SD male rats were selected to make wounds of 3.0 cm×3.0 cm in the back and sewed on the inner edge of the wound with wire coils.The rats were divided into 3 groups according to the random number table method,which were general wound group,chronic wound group and micropar skin pulp group.There were 20 rats in each group.The general wound group was treated with anti-infective therapy and the routine dressing change after the wound was caused by the back.The chronic wound group was treated with anti-infection treatment and routine dressing change after the formation of the back wound.Chronic wound formation was completed by intramuscular injection of hydrocortisone for 7 days.After the formation of the wound on the back of the micropar skin pulp group,anti-infection treatment and the routine dressing change were given,intramuscular injection of hydrocortisone was performed for 7 days in a row to interfere with the formation of the chronic wound,and the microne was prepared from the skin on the lateral side of the right thigh of the rats and injected under granulation tissue.The observation of wound surface was started next day after the completion of modeling,which was set as the observation day.The wound healing of each group was observed on the 7th,14th,21st,28th day of observation and the wound healing rate was calculated.Granulation tissues on 14th day of observation were retained and observed for hematoxylin-eosin staining and CD31 immunohistochemical staining.The distribution of new capillaries was observed under hematoxylin-eosin staining and the expression of CD31,microvessel density of the wound were observed by CD31 immunohistochemical staining.Data were analyzed with one-way analysis of variance and LSD-t test.Results On the 14th day of observation,the wound of the general wound group was significantly reduced,the skin climbing of the chronic wound group was

关 键 词：大鼠 创伤和损伤 伤口愈合 微粒皮浆 慢性创面 微血管密度 

分 类 号：R47[医药卫生—护理学]