血清2-ME、胎盘组织儿茶酚-O-甲基转移酶蛋白表达与重度子痫前期孕妇病情严重程度的相关性研究  

Correlation between expression of serum 2-ME and placental tissue catechol-O-methyltransferase protein and severity of severe preeclampsia in pregnant women

作　　者：李喜梅 毛蕾蕾 冯泽蛟 林丽 LI Xi-mei;MAO Lei-lei;FENG Ze-jiao;LIN Li(Department of Obstetrics and Gynecology,Wenzhou Central Hospital,Wenzhou,Zhejiang 325000,China)

机构地区：[1]温州市中心医院妇产科,浙江温州325000

出　　处：《中国卫生检验杂志》2021年第4期489-492,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的探讨血清2-甲氧雌二醇(2-ME),胎盘组织儿茶酚-O-甲基转移酶(COMT)蛋白表达与重度子痫前期(EOSPE)孕妇病情严重程度的相关性。方法选择2018年1月-2019年7月本院接诊重度子痫前期孕妇57例为观察组,同期选择60例正常孕妇作为对照组。用酶联免疫吸附试验测定2组孕妇血清2-ME表达水平,经免疫组化法、蛋白质印迹法测定胎盘组织COMT蛋白表达,并用RT-PCR法测定胎盘组织COMT mRNA表达水平。Pearson相关性分析法分析血清2-ME、胎盘组织COMT蛋白表达与重度子痫前期孕妇病情严重程度的相关性。结果观察组血清2-ME表达低于对照组(P<0.05);观察组胎盘组织COMT.COMT mRNA蛋白相对表达量与对照组比较差异无统计学意义(P>0.05);Pearson相关性分析显示,血清2-ME表达水平与重度子痫前期孕妇的病情呈负相关(r=-0.649,P<0.05);胎盘组织COMT蛋白表达水平和重度子痫前期孕妇的病情无相关性(r=-0.027,P>0.05)。结论重度子痫前期孕妇的发生、进展与胎盘组织COMT蛋白表达无关,但与血清2-ME指标密切相关,可作为临床评估的重要依据。Objective To explore the correlation between the expression of serum 2-ME(2-methoxyestradiol)and placental tissue COMT(catechol-O-methyltransferase)protein and the severity of severe pre-eclampsia pregnant women.Methods A total of 57 pregnant women with severe preeclampsia admitted to our hospital from January 2018 to July 2019 were selected as the observation group.During the same period,60 normal pregnant women were selected as the control group.Enzyme-linked immunosorbent assay was used to determine the expression of 2-ME in the serum of the two groups of pregnant women.The expression of COMT protein in the placenta tissue was determined by immunohistochemistry and western blotting,and the expression of COMT mRNA in the placenta tissue was determined by RT-PCR.Pearson correlation analysis was used to analyze the correlation between serum 2-ME and COMT protein expression in placental tissues and the severity of severe pre-eclampsia pregnant women.Results The expression of serum 2-ME in the observation group was lower than that in the control group(P<0.05);the relative expression of COMT and COMT mRNA proteins in the placenta tissue of the observation group was lower than that in the control group,but the difference was not statistically significant(P>0.05);Pearson correlation analysis showed that serum 2-ME expression level was significantly negatively correlated with the condition of pregnant women with severe preeclampsia(r=-0.649,P<0.05);there was no statistical significance on the correlation between the expression of COMT protein in placental tissue and the condition of pregnant women with severe preeclampsia(r=-0.027,P>0.05).Conclusion The expression of 2-ME in serum of pregnant women with severe preeclampsia was significantly reduced,while the expression of COMT protein was not significantly changed.It is suggested that the occurrence and progression of pregnant women with severe preeclampsia are not related to the expression of COMT protein in the placenta tissue,but are closely related to the serum 2-M

关 键 词：血清2-甲氧雌二醇 胎盘组织儿茶酚-O-甲基转移酶蛋白 重度子痫前期 病情严重程度 

分 类 号：R714.244[医药卫生—妇产科学]