常用工具病毒在针刺脑效应研究中的应用前景  

Application prospect of common viral tracers in study on brain effect of acupuncture

作　　者：张悦 王俊英[1] 李少源[1] 李亮[1] 陈瑜 荣培晶[1] ZHANG Yue;WANG Jun-ying;LI Shao-yuan;LI Liang;CHEN Yu;RO NG Pei-jing(Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院针灸研究所,北京100700

出　　处：《中国针灸》2021年第2期205-209,共5页Chinese Acupuncture & Moxibustion

基　　金：国家重点研发计划“中医药现代化研究”重点专项:2018YFC1705800;国家自然科学基金面上项目:81674072;国家自然科学基金中德合作研究项目:GZ1236。

摘　　要：探讨工具病毒及其介导的功能元件在针刺脑效应研究中应用的可行性及前景。本文从工具病毒入手,介绍了用于解析特定神经环路结构的工具病毒及其介导的探针、光/化学遗传学技术、Cre-LoxP系统等。工具病毒及其介导的功能元件,既可特异性标记神经细胞或神经环路,又可干预特定类型神经元或核团的功能,能解决传统神经示踪方法仅可描绘一个脑区神经元的形态及脑区之间简单的投射,且有信号间接、非特异性吸收等问题,将其引入针灸实验是解码针刺脑效应机制的重要手段,为深入解析针刺脑效应的神经生物学机制提供了一种在体、实时、直观的新方法。The feasibility and prospect of viral tracers and mediating functional components are explored in study on brain effect of acupuncture. In the paper, proceeding with viral tracers, the viral tracers used to analyze the structure of specific neural circuits are introduced, as well as their mediated probes, optical/chemical genetics techniques, Cre-LoxP systems, etc. The viral tracers and their functional components can not only mark specifically nerve cells or neural circuits, but also interfere with the function of specific types of neurons or nuclei. They solve some disadvantage of traditional nerve tracing method that only describes the morphology of neurons of one brain region and the simple projection among brain regions, and the indirect and non-specific absorption. The viral tracers and their functional components play the important approach to decoding the mechanism on brain effect of acupuncture when introduced in experimental acupuncture so as to provide an in vivo, real-time and intuitive novel method for a further analysis of neurobiological mechanism on brain effect of acupuncture.

关 键 词：针刺脑效应 工具病毒 探针 光遗传学 化学遗传学 

分 类 号：R245[医药卫生—针灸推拿学]