g-C_(3)N_(4)@C-TiO_(2)纳米颗粒增强可见光驱动的体外光动力灭活HL60细胞的试验研究  

An Experimental Study Based on g-C_(3)N_(4)@C-TiO_(2) Nanoparticles to Enhance Visible-light-driven Photodynamic Therapy for HL60 Cells in vitro

作　　者：方杰 王健[1] 张启云 李淼淼 肖睦沧 艾保全[1] 熊建文[1] FANG Jie;WANG Jian;ZHANG Qiyun;LI Miaomiao;XIAO Mucang;AI Baoquan;XIONG Jianwen(School of Physics and Telecommunication Engineering,South China Normal University,Guangzhou 510006,China)

机构地区：[1]华南师范大学物理与电信工程学院,广州510006

出　　处：《激光生物学报》2021年第1期43-51,共9页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(61072029);广州市科技计划项目(2014J4100049)。

摘　　要：通过化学气相沉积法制备了g-C_(3)N_(4)@C-TiO_(2)纳米颗粒,利用X射线衍射(XRD)、荧光光谱(FS)、透射电子显微镜(TEM)、紫外-可见吸收光谱(UV-Vis)、能谱(EDS)等对纳米颗粒进行表征。分别研究了暗室条件及光照条件下g-C_(3)N_(4)@C-TiO_(2)纳米颗粒对HL60细胞的作用效果。采用CCK-8法探究了一系列质量浓度梯度的纳米颗粒处理HL60细胞后的存活率,并且通过荧光探针标记技术检测细胞内活性氧水平。试验结果表明,在C-TiO_(2)表面包裹g-C_(3)N_(4),可将TiO_(2)可吸收的光波长范围拓展至可见光波段。制备的g-C_(3)N_(4)@C-TiO_(2)粒径在10~20 nm,满足其进入细胞的尺寸要求。暗毒性试验表明g-C_(3)N_(4)@C-TiO_(2)纳米颗粒在暗室条件下对细胞的毒性较小,证明了其具有良好的生物相容性。同时,与TiO_(2)和C-TiO_(2)纳米颗粒处理组相比,g-C_(3)N_(4)@C-TiO_(2)处理组的光动力灭活效率高达(76.5±1.9)%,表明其可能成为治疗白血病的潜在光敏剂。C-doped TiO_(2) nanoparticles combined with g-C_(3)N_(4) were prepared by chemical vapor deposition,using X-ray diffraction,fluorescence spectroscopy,transmission electron microscopy,UV-Vis absorption spectroscopy and energy dispersive spectrometer to characterize nanoparticles.The effects of g-C_(3)N_(4)@C-TiO_(2) nanoparticles on HL60 cells in vitro were explored in dark and under light irradiation.Cell Counting Kit-8(CCK-8)method was used to investigate the viability of HL60 cells treated with nanoparticles with a series of concentration gradients,and the level of intracellular reactive oxygen species was detected via fluorescent probe labeling technology.The experimental results showed that by modifying g-C_(3)N_(4) on the surface of C-TiO_(2),the absorption wavelength range of TiO2 could be extended to the visible band.The particle size of g-C_(3)N_(4)@C-TiO_(2) prepared was about 10~20 nm,which met the size requirement of entering cells.Dark toxicity experiment proved that g-C_(3)N_(4)@C-TiO_(2) nanoparticles were less toxic to cells under darkroom conditions,which suggested they had good biocompatibility.Meanwhile,compared with the groups treated with TiO2 and C-TiO_(2) nanoparticles,the photocatalytic inactivation efficiency of the group treated with g-C_(3)N_(4)@C-TiO_(2) was as high as(76.5±1.9)%under light irradiation,indicating its potential as a potential photosensitizer for the treatment of leukemia.

关 键 词：光动力治疗 g-C_(3)N_(4)@C-TiO_(2) 光敏剂 HL60细胞 肿瘤细胞凋亡 

分 类 号：R454.2[医药卫生—治疗学]