γ射线辐射诱导水稻突变体的基因组变异分析  

Genome-wide Analysis of Radiation-induced Mutations in Rice Mutant

作　　者：张莉 张勇 张渊海 张逸妍 解涛 彭选明 杨震 ZHANG Li;ZHANG Yong;ZHANG Yuanhai;ZHANG Yiyan;XIE Tao;PENG Xuanming;YANG Zhen(Hunan Institute of Nuclear Agriculture Sciences and Space Mutagenesis Breeding,Changsha 410125,China;Hunan Engineering Technology Center for Agricultural Biological Irradiation,Changsha 410125,China)

机构地区：[1]湖南省核农学与航天育种研究所,长沙410125 [2]湖南省农业生物辐照工程技术研究中心,长沙410125

出　　处：《激光生物学报》2021年第1期59-66,共8页Acta Laser Biology Sinica

基　　金：湖南省农业科技创新资金项目(2018ZD01-5,2019LS07,2020CX45-1);国家十三五重点研发专项项目(2016YFD0102103);国家自然科学基金项目(11205055)。

摘　　要：辐射诱变技术广泛应用于水稻种质资源创新。为了明确辐射诱导突变的分子机制,采用二代高通量测序技术对水稻品种黄华占经辐射诱导获得的一个突变体湘辐1821进行全基因组变异分析。与参考序列蜀恢R498相比,在黄华占和湘辐1821中分别检测到758215和799434个单核苷酸多态性位点(SNPs),142313和147686个插入缺失(InDels),16775和18382个结构变异(SVs)以及16614和17658个拷贝数变异(CNVs)。黄华占和湘辐1821的SNPs转换与颠换的比率约为2.5,这两种基因型SNPs以转换为主要类型。在黄华占和湘辐1821的InDels中短序列InDels远多于长序列,说明点突变为主要突变方式。拷贝数减少是黄华占和湘辐1821的主要拷贝数变异方式。同时对黄华占和湘辐1821重测序数据整合差异比较分析,两种基因型的差异SNPs有86163个,InDels有88777个。对差异SNPs位点所在基因进行基因本体(GO)分析,3092个候选基因中70%位于生物学过程中,该研究结果将对湘辐1821表型分析具有重要参考意义,同时为辐射诱导突变机制提供新的见解。Radiation-induced mutation has been efficiently used for rice germplasm innovation.In order to characterize its molecular mechanisms,whole genome-wide sequencing was performed to analyze the variations inγ-radiationinduced rice mutant Xiangfu 1821 and the wildtype Huanghuazhan.Compared with the reference R498,a total of 758215 and 799434 single nucleotide polymorphisms(SNPs),142313 and 147686 insertions/deletions(InDels),16775 and 18382 structural variations and 16614 and 17658 copy number variations were identified in Huanghuazhan and Xiangfu 1821,respectively.The ratio of transitions to transversions was~2.5 in Huanghuazhan and Xiangfu 1821,the transitions was the main type.There was a greater number of shorter InDels than longer InDels,suggesting that point mutation was the dominant type of mutations in Huanghuazhan and Xiangfu 1821.Copy number reduction was the main copy number variation type of Huanghuazhan and Xiangfu 1821.Through an integrative analysis of sequence variations between Huanghuazhan and Xiangfu 1821,we identified 86163 SNPs and 88777 InDels.The genes that included SNPs between Huanghuazhan and Xiangfu 1821 were used for GO analysis.Among the 3092 candidate genes,70%of them were involved in biological processes.These results will help to understand the phenotype of Xiangfu 1821 and provide novel insights into mechanism of the mutation breeding.

关 键 词：水稻突变体 辐射诱变 基因组测序 DNA多态性 基因本体分析 

分 类 号：Q319[生物学—遗传学]