人类miR-146a-5p靶基因预测及生物信息学分析  

Target Gene Prediction and Bioinformatic Analysis of hsa-miR-146a-5p

作　　者：王道[1] 朱洪怡[2] 楚毅[2] WANG Dao;ZHU Hongyi;CHU Yi(The Second Xiangya Hospital,Central South University,Department of Obstetrics and Gynecology,Changsha 410011,China;The Second Xiangya Hospital,Central South University,Department of Gastroenterology,Changsha 410011,China)

机构地区：[1]中南大学湘雅二医院妇产科,长沙410011 [2]中南大学湘雅二医院消化内科,长沙410011

出　　处：《激光生物学报》2021年第1期75-83,共9页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(31670178);湖南省自然科学基金项目(2017JJ3436)。

摘　　要：微小RNA(miRNA)是疾病发生发展过程中的调节剂,但其在炎症反应、免疫应答,特别是肿瘤中的调控机制仍然未知。本研究先对人类miR-146a-5p进行定位,分析序列保守性,挖掘其在不同组织中表达特征;同时,将Targetscan7.1、miRDB、mirDIP和DIANA TOOLS数据库预测的人类miR-146a-5p靶基因取交集,并通过String11.0构建了蛋白相互作用(PPI)网络;利用Omicshare平台和KOBAS数据库进行基因本体(GO)注释分析和京都基因与基因组百科全书(KEGG)通路富集分析。结果显示:人类miR-146a-5p成熟序列的保守性高,推测其具有重要生物学功能;73个靶基因涉及到结构分子活性、催化活性以及转录因子活性配体的激活,并且参与机体代谢、信号传导、正负向调控等生物学过程;还与Toll样受体(TLRs)信号、核转录因子-κB(NF-κB)以及视黄酸诱导基因I样受体(RLRs)等相关信号通路相关联。本研究初步阐明了人类miR-146a-5p通过调控靶基因在肿瘤发生、免疫应答和炎症刺激等疾病发生发展过程起着重要作用,人类miR-146a-5p将可能成为潜在的生物标记物和治疗靶标。MicroRNA(miRNA)is a key regulator in the processes of disease development,however its regulatory mechanism in inflammatory reaction,immune response,especially in tumors is still unknown.In this study,we firstly predicted and analyzed the location,sequence conservation of hsa-miR-146a-5p aiming to get deep insight into the expression level in different tissues.Meanwhile,the target genes of hsa-miR-146a-5p were predicted by Targetscan7.1,miRDB,mirDIP and DIANA TOOLS.Additionally,protein-protein interaction(PPI)network was constructed through String 11.0.Gene ontology(GO)and Kyoto encyclopedia of genes and genome(KEGG)pathway enrichment analysis were performed on Omicshare platform and KOBAS databases.The results have indicated that the mature sequences of hsa-miR-146a-5p was highly conserved,with important biological functions.The 73 target genes intersect mainly involved transcriptional activity,structural molecular activity,catalytic activity,and activation of transcription factor active ligands.The significantly enriched biological processes included metabolism,signal transduction,positive or negative regulation,and they were related to Toll-like receptors pathway,NF-κB pathway,RIG-I like receptors and other signaling pathways.Our study was preliminarily conducted to elucidate that hsa-miR-146a-5p participates in tumorigenesis,immune response and inflammatory stimulation by regulating target genes,which would be a novel biomarker and therapeutic target.

关 键 词：人类 miR-146a-5p 靶基因 生物信息学 治疗靶标 

分 类 号：Q344[生物学—遗传学]