藿苏养胃口服液治疗胃癌的作用机制研究  

Mechanisms of Huosu Yangwei Oral Liquid in treatment of gastric cancer

作　　者：赵堃鹏 张惠惺 张彩云 刘悦涵 方盛泉 倪红梅[2] ZHAO Kunpeng;ZHANG Huixing;ZHANG Caiyun;LIU Yuehan;FANG Shengquan;NI Hongmei(Institute of Interdisciplinary Integrative Medicine Research,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;School of Basic Medical Sciences,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Department of Gastroenterology,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China)

机构地区：[1]上海中医药大学交叉科学研究院,上海201203 [2]上海中医药大学基础医学院,上海201203 [3]上海中医药大学附属岳阳中西医结合医院消化科,上海200437

出　　处：《上海中医药大学学报》2021年第1期112-120,共9页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：上海市自然科学基金资助项目(20ZR1459300);上海中医药大学附属岳阳中西医结合医院院级基金重点资助项目(2019YYZ01);上海申康医院发展中心临床研究培育项目(SHDC12018X30)。

摘　　要：目的:探讨藿苏养胃口服液治疗胃癌的作用机制。方法:通过DrugBank、TCMSP、TCMIP、PubChem数据库查找藿苏养胃口服液的主要化学成分,获得其靶标及疾病信息,通过GeneCards、SwissTargetPrediction、MalaCards、CTD及TTD数据库对胃癌对应靶点进行预测筛选。利用Cytoscape软件构建"中药-化学成分-潜在靶点"网络图,通过靶蛋白构建蛋白相互作用(PPI)网络,使用DAVID、IPA数据库进行GO功能和KEGG通路分析,并进行体外实验验证。结果:藿苏养胃口服液中共筛选得到350个化学成分、182个药物作用靶点、4 346个成分-靶点配对,构建其在胃癌中的成分-靶点网络,筛选得出HRH1、COL1A1、HMOX1、LTA4H、GSTM2、GABRA6及AKR1C1 7个核心靶点。qRT-PCR检测结果显示,与对照组相比,藿苏养胃口服液组7个核心靶点的基因表达水平显著改变。结论:藿苏养胃口服液可能通过HRH1、COL1A1、HMOX1、LTA4H、GSTM2、GABRA6及AKR1C1等靶点治疗胃癌。Objective: To explore the mechanisms of Huosu Yangwei(HSYW) Oral Liquid in the treatment of gastric cancer. Methods: The main chemical components of HSYW Oral Liquid were searched from DrugBank, TCMSP, TCMIP and PubChem databases, and their targets and disease information were obtained. The corresponding targets of gastric cancer were predicted and screened out through databases such as GeneCards, SwissTargetPrediction, MalaCards, CTD and TTD. Cytoscape software was used to construct the network diagram of "drug-chemical component-potential target". Protein-protein interaction(PPI)network was constructed by analyzing the target proteins. GO function and KEGG pathway analyses were performed by DAVID and IPA databases, and the experiment in vitro was performed for verification. Results: A total of 350 chemical components, 182 drug targets and 4 346 component-target pairs were screened out from HSYW Oral Liquid. Seven key targets including HRH1, COL1 A1, HMOX1, LTA4 H, GSTM2, GABRA6 and AKR1 C1 were screened out by constructing the component-target network in gastric cancer. The results of qRT-PCR showed that the expression levels of the 7 core targets were significantly changed in the HSYW Oral Liquid group compared with the control group. Conclusion: HSYW Oral Liquid may treat gastric cancer through HRH1,COL1 A1,HMOX1,LTA4 H,GSTM2,GABRA6 and AKR1 C1.

关 键 词：藿苏养胃口服液 胃癌 网络药理学 胃癌细胞 

分 类 号：R285[医药卫生—中药学]