离子色谱-脉冲安培检测法测定洋常春藤游离糖成分含量  

Content Determination of Free Sugar Components in Hedera Helix by Ion Chromatography-Integrated Pulsed Amperometric Detection

作　　者：史万忠[1] 栾绍斋 倪力军[3] SHI Wanzhong;LUAN Shaozhai;NI Lijun(Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai,China 201203;Unit 93,Army 71239 of PLA,Yingkou,Liaoning,China 115002;School of Chemistry and Molecular Engineering,East China University of Science and Technology,Shanghai,China 200237)

机构地区：[1]上海中医药大学附属曙光医院,上海201603 [2]中国人民解放军71239部队93分队,辽宁营口115002 [3]华东理工大学化学与分子工程学院,上海200237

出　　处：《中国药业》2021年第5期54-58,共5页China Pharmaceuticals

摘　　要：目的建立测定洋常春藤中游离糖成分含量的离子色谱-脉冲安培检测法。方法色谱柱采用Thermo DionexCarboPac PA1分析柱(250 mm×4 mm)和Thermo DionexCarboPac PA1保护柱(50 mm×4 mm),流动相为超纯水(A)-50 mmol/L NaOH和1 mol/L NaAc溶液(B)-100 mmol/L NaOH溶液(C),梯度洗脱(0~13.00 min时82%A、18%C,13.10~22.00 min时70%A、30%C,22.10~31.00 min时67%A、15%B、18%C,31.10~32.00 min时82%A、18%C),流速为1.0 m L/min,柱温为25℃,进样量为10μL。采用四电位脉冲安培检测器检测。结果海藻糖、鼠李糖、阿拉伯糖、半乳糖、D-无水葡萄糖、D-木糖、D-果糖、D-核糖、蔗糖、乳糖、D-半乳糖醛酸、D-葡萄糖醛酸在各自的质量浓度范围内线性关系良好;阿拉伯糖、D-无水葡萄糖、D-果糖、蔗糖的平均回收率分别为99.21%,97.84%,97.32%,96.20%,RSD分别为3.46%,3.85%,2.94%,3.28%(n=6)。结论该方法结果准确、可靠,重复性好,可用于洋常春藤中12种游离糖成分含量的分析和质量控制。Objective To establish an ion chromatography-integrated pulsed amperometric detection method for the content determination of free sugar components in Hedera helix.Methods The chromatographic column was Thermo DionexCarboPac PA1 analytical column(250 mm×4 mm),and Thermo DionexCarboPac PA1 guard columns(50 mm×4 mm),the mobile phase was ultra-pure water(A)-50 mmol/L NaOH and 1 mol/L NaAc solution(B)-100 mmol/L Na OH(C)with gradient elution(0-13.00 min,82%A and 18%C;13.10-22.00 min,70%A and 30%C;22.10-31.00 min,67%A,15%B and 18%C;31.10-32.00 min,82%A and 18%C),the flow rate was 1.0 m L/min,the column temperature was 25℃,and the injection volume was 10μL.The pulsed amperometric detector was operated with aquadruple-potential waveform for the detection.Results Trehalose,rhamnose,arabinose,galactose,D-glucose,D-xylose,D-fructose,D-ribose,sucrose,lactose,D-galacturonic acid and D-glucuronic acid showed a good linear relationship within the corresponding concentration range.The average recoveries of arabinose,D-glucose,D-fructose and sucrose were 99.21%,97.84%,97.32%,96.20%with RSDs of 3.46%,3.85%,2.94%and 3.28%(n=6).Conclusion The method is accurate,reliable and reproducible,which can be used for the content determination and quality control of free sugar components in Hedera helix.

关 键 词：离子色谱法 脉冲安培检测法 洋常春藤 游离糖 含量测定 

分 类 号：R932[医药卫生—生药学] R2841[医药卫生—药学]