1株猫杯状病毒的分离鉴定及其ORF2序列的遗传演化分析  

Isolation and identification of a feline calicivirus strain and phylogenetic analysis of its ORF2 sequence

作　　者：李鑫[1] 杨德全[1] 葛菲菲[1] 沈海潇 刘健[1] 鞠厚斌[1] 王建[1] 赵洪进[1] LI Xin;YANG Dequan;GE Feifei;SHEN Haixiao;LIU Jian;JU Houbin;WANG Jian;ZHAO Hongjin(Shanghai Animal Disease Control Center,Shanghai 201103,China)

机构地区：[1]上海市动物疫病预防控制中心,上海201103

出　　处：《畜牧与兽医》2021年第2期92-96,共5页Animal Husbandry & Veterinary Medicine

基　　金：绿色农产品产业发展技术创新应用项目(沪农科创字(2019)第3-3号)。

摘　　要：为了解猫杯状病毒形态特征及遗传演化情况,采用F81细胞从患病宠物猫的鼻拭子样品中分离获得1株猫杯状病毒(feline calicivirus,FCV),命名为SH1。经电镜观察,病毒粒子呈球形,无囊膜,符合FCV的形态特征。采用RT-PCR方法扩增了该毒株的全基因组,并进行了序列测定和衣壳蛋白基因(ORF2)序列的分析。结果显示:分离株与国内外参考株的ORF2序列的核苷酸和氨基酸同源性分别为74.1%~79.7%和84.5%~90.9%;ORF2基因的遗传演化分析显示,30株FCV毒株形成两大分支,即基因群Ⅰ和Ⅱ,分离株属于基因群Ⅰ;进一步分析发现,基因群Ⅰ和Ⅱ主要在377、539和557氨基酸位点存在差异,基因群Ⅰ和Ⅱ分别为N、A、G和K、V、S。研究结果为FCV感染的防控提供科学依据。In this study, F81 cells were used to isolate a feline calicivirus(FCV) strain from a nasal swab sample of a sick domestic cat, and the strain was named SH1. Observation by an electron microscope showed the virus particles to be spherical, without capsule, and to conform to the morphological characteristics of FCV. The complete genome of the strain was amplified by RT-PCR and was analyzed. ORF2 gene sequence analysis showed that the nucleotide and amino acid homology of the ORF2 sequences of the strain at home and abroad with our isolated strain were 74.1%-79.7% and 84.5%-90.9%, respectively. Phylogenetic analysis of the ORF2 gene showed that 30 FCV isolates clustered two large branches, namely, genogroups Ⅰ and Ⅱ, and our isolate belonged to genogroup Ⅰ. Further analysis found that there were three major amino acid differences(377, 539, and 557) between genogroups Ⅰ and Ⅱ, which were N, A, G and K, V, S, respectively.

关 键 词：猫杯状病毒 分离鉴定 衣壳蛋白基因 遗传演化 

分 类 号：S852.65[农业科学—基础兽医学]