猪传染性胸膜肺炎与猪肺疫二联亚单位疫苗的免疫效果评价  

Evaluation of the immune effect of bivalent vaccine containing the subunit antigens of infectious pleuropneumonia and swine pneumonic pasteurellosis

作　　者：姚景丽 武静桥 范真玮 李丹 蓝肇煜 王小月 赵微 陈婷 何敬文 金天明 YAO Jingli;WU Jingqiao;FAN Zhenwei;LI Dan;LAN Zhaoyu;WANG Xiaoyue;ZHAO Wei;CHEN Ting;HE Jingwen;JIN Tianming(College of Animal Science and Veterinary Medicine,Tianjin Agricultural University/Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry,Tianjin 300384,China)

机构地区：[1]天津农学院动物科学与动物医学学院/天津市农业动物繁育与健康养殖重点实验室,天津300384

出　　处：《畜牧与兽医》2021年第2期102-109,共8页Animal Husbandry & Veterinary Medicine

基　　金：国家自然基金项目(31572492);天津市科技计划项目(高端兽药先进制造科技重大专项)(17ZXGSNC00030);天津市农委农业科技示范推广项目(201601380);天津市兽医生物技术优秀科研创新团队资助项目(TD12-5019);天津市科技支撑项目(19ZXBTSN00250)。

摘　　要：为了评价猪传染性胸膜肺炎与猪肺疫二联亚单位疫苗(由多杀性巴氏杆菌外膜蛋白H(OmpH)质粒导入胸膜肺炎放线杆菌(APP)菌影制备)的免疫效果,于某规模化猪场选取30头无上述病原菌和抗体的4周龄(w)仔猪,随机分为安全评估组和试验组进行免疫,试验组包括APP组、OmpH组、APP+OmpH联苗组,分别于4、6 w肌肉注射2 mL/头相应疫苗,采取4、6、9 w外周血,用流式细胞术、ELISA、血常规、血生化试验分析疫苗免疫后,相关免疫因子、免疫细胞及免疫球蛋白的变化。结果显示:APP+OmpH组外周血中CD3^(+)、CD8^(+)细胞增加数量比APP、OmpH组显著增多(P<0.05);血清抗体结果显示,APP+OmpH组结果优于APP和OmpH组;APP+OmpH组的外周血白细胞数、淋巴细胞数、中性粒细胞数、单核细胞数在二免后增多,白蛋白、球蛋白亦明显增加,且APP+OmpH组增加数量多于APP和OmpH组。结论:APP+OmpH组能更好地刺激机体细胞免疫和体液免疫反应,进而发挥免疫保护效果。The experiment was to evaluate the immune effect of bivalent vaccine containing the subunit antigens of infectious pleuropneumonia and swine pneumonic pasteurellosis;is the vaccine was prepared by introducing the Pasteurella multocida outer membrane protein H(OmpH) plasmid into the Actinobacillus pleuropneumoniae(APP) bacterial ghosts. 30 4-week-old piglets without the above pathogens and antibody interference were selected on a large-scale pig farm, were randomly divided into the safety evaluation group and the experimental groups, and were immunized.The experimental groups were the APP and OmpH ones, and the one with combined APP-and-OmpH bivalent vaccine. Two ml of the vaccine was intramuscularly injected into each experimental animal at 4 and 6 weeks of age, respectively. Then, peripheral blood samples were collected from the experimental animals when they were at 4, 6 and 9 weeks of age. Flow cytometry, ELISA assay, blood routine tests and blood biochemical analysis were performed to analyze and evaluate the changes in related immune factors, immune cells and immunoglobulins after vaccination. The results showed that, in the APP + OmpH bivalent vaccine group, the number of CD3^(+) and CD8^(+) cells in the peripheral blood increased significantly, as compared with the APP and OmpH vaccine groups(P<0.05). The results of serum antibody analysis showed that the APP+OmpH bivalent vaccine group were better than the APP and OmpH monovalent vaccine groups;the number of leukocytes, lymphocytes, neutrophils, and monocytes in the peripheral blood of the APP + OmpH bivalent vaccine group increased after the second immunization,and its albumin and globulin also increased significantly. The bivalent vaccine group increased more in these factors than the APP and Omp H vaccine groups did. To conclude,APP + Omp H bivalent vaccine stimulated the body’s cellular immunity and humoral immune response more effectively,thereby exerting the immune protection effect.

关 键 词：猪传染性胸膜肺炎 猪肺疫 亚单位疫苗 免疫效果 

分 类 号：S852.6[农业科学—基础兽医学]