PCOS合并甲状腺抗体阳性患者外周血T细胞亚型和CD4^(+)CD25^(+)调节性T细胞研究  

Analysis of peripheral blood T cell subtypes and CD4^(+)CD25^(+)regulatory T cells in PCOS combined with thyroid antibody patients

作　　者：胡伟娇 娄江涛 周俊 殷一红 HU Weijiao;LOU Jiangtao;ZHOU Jun;YIN Yihong(Clinical Laboratory,Ningbo Traditional Chinese Medicine Hospital,Ningbo 315012,China)

机构地区：[1]宁波市中医院检验科,315012

出　　处：《浙江医学》2021年第4期395-400,共6页Zhejiang Medical Journal

摘　　要：目的分析多囊卵巢综合征(PCOS)合并甲状腺抗体阳性患者外周血T细胞亚型分布情况和CD4^(+)CD25^(+)调节性T细胞数量及功能,探讨患者的机体免疫状态。方法选取PCOS患者、PCOS合并甲状腺抗体阳性患者、甲状腺抗体阳性患者及身体健康的女性体检者(正常对照)各50例,采用免疫发光法检测甲状腺过氧化物酶抗体(TPOAb)、甲状腺球蛋白抗体(TGAb)、促卵泡生成素(FSH)、促黄体生成素(LH)、睾酮(T)和胰岛素(INS)水平,全自动生化分析仪检测空腹血糖,爱威全自动粪便分析仪检测粪球杆菌比;流式细胞术检测外周血T细胞亚型分布及CD4^(+)CD25^(+)调节性T细胞比例;采用实时荧光定量PCR检测Th1、Th2的效应因子IFN-γ、IL-4以及反映CD4^(+)CD25^(+)调节性T细胞功能的叉状头/翅膀状螺旋转录因子(Foxp3)mRNA表达情况;Pearson法分析Foxp3 mRNA表达水平与其它指标的相关性。结果与正常对照组相比,甲状腺抗体阳性组患者除粪便球杆菌比升高外(P<0.05),BMI、胰岛素抵抗指数(HOMA-IR)、LH/FSH、T均无统计学差异(均P>0.05)。与甲状腺抗体阳性组相比,PCOS组及PCOS合并甲状腺抗体阳性组患者的BMI、HOMA-IR、LH/FSH及T均升高(均P<0.05),而PCOS组粪便球杆菌比降低(P<0.05)。与PCOS组相比,PCOS合并甲状腺抗体阳性组患者BMI、HOMA-IR、LH/FSH及粪便球杆菌比均升高(均P<0.05)。与正常对照组相比,甲状腺抗体阳性组、PCOS组和PCOS合并甲状腺抗体阳性组患者的CD4^(+)T细胞比例及CD4^(+)T细胞/CD8^(+)T细胞比例均升高(均P<0.05),CD8^(+)T细胞及CD4^(+)CD25^(+)调节性T细胞比例均降低(均P<0.05);与PCOS组相比,PCOS合并甲状腺抗体阳性组患者CD4^(+)T细胞比例及CD4^(+)T细胞/CD8^(+)T细胞比例升高,CD8^(+)T细胞、CD4^(+)CD25^(+)调节性T细胞比例降低(均P<0.05)。与正常对照组相比,甲状腺抗体阳性组、PCOS组及PCOS合并甲状腺抗体阳性组患者IFN-γmRNA和Foxp3 mRNA表达均下调(均P<0Objective Analysis of PCOS and PCOS with thyroid antibody patients peripheral blood T cell subtype distribution and the CD4^(+)CD25^(+)regulatory T cell’s number and function,reveal the immune status of PCOS with thyroid antibody patients.Methods Fifty PCOS patients,50 PCOS with thyroid antibody positive patients,50 thyroid antibody positive patients and 50 healthy physical examination patients were selected respectively.Immunoluminescence was used to detect anti-thyroglobulin antibody(TGAb),antithyroid peroxidase autoantibody(TPOAb),LH,FSH,T and INS.The fasting blood glucose was detected by automatic biochemical analyzer.Aiwei automatic stool analyzer was used to detect fecal cocci bacillus ratio.Flow cytometry was used to detect the distribution of T cell subsets and the percentage of CD4^(+)CD25^(+)regulatory T cells.Real-time fluorescence quantitative PCR was used to detect the expression of Th1 and Th2 effector factors IFN-γand IL-4,as well as fork head box protein p3(Foxp3),which reflects the function of CD4^(+)CD25^(+)regulatory T cells.Pearson method was used to analyze the correlation between Foxp3 gene expression and other indicators.Results Compared with the normal control group,the fecal cocci bacillus ratio increased in the thyroid antibody positive group(P<0.05),and there was no significant change in other indicators(P>0.05).Compared with the thyroid antibody positive group,BMI,homa-IR,LH/FSH and T of patients in PCOS group and PCOS with thyroid antibody positive group all increased(P<0.05),while the fecal cocci bacillus ratio in PCOS group decreased(P<0.05).Compared with the PCOS group,the ratio of BMI,HOMA-IR,LH/FSH and the fecal cocci bacillus ratio in the PCOS with thyroid antibody positive group were increased(P<0.05).The results of flow cytometry showed that compared with the number of CD4 T cells and the ratio of CD4/CD8 in normal control group,the thyroid antibody positive group,PCOS group and PCOS with thyroid antibody positive group were gradually increased(P<0.05),while the number of C

关 键 词：多囊卵巢综合征 T细胞亚型 调节性T细胞 甲状腺球蛋白抗体 甲状腺过氧化物酶抗体 

分 类 号：R711.75[医药卫生—妇产科学]