白藜芦醇/富勒烯纳米组装体的制备——在胆管支架抗炎涂层中的应用评估  

Preparation of Resveratrol/Fullerene Nano-assembly and Its Application in Anti-inflammatory Coating of Bile Duct Stent

作　　者：高建 刘永佳 戚晓亮[3] 徐汪节 乔中东[1] GAO Jian;LIU Yongjia;QI Xiaoliang;XU Wangjie;QIAO Zhongdong(School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China;Instrumental Analysis Center,Shanghai Jiao Tong University,Shanghai 200240,China;Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University Schole of Medicine,Shanghai 200011,China)

机构地区：[1]上海交通大学生命科学技术学院,上海200240 [2]上海交通大学分析测试中心,上海200240 [3]上海交通大学医学院附属第九人民医院,上海200011

出　　处：《实验室研究与探索》2021年第2期58-62,共5页Research and Exploration In Laboratory

摘　　要：采用白藜芦醇(Res)和富勒烯(C60)结合制备Res/C60纳米组装体并验证其安全性和抗炎性。通过扫描电子显微镜(SEM)、拉曼光谱和X射线衍射研究了纳米组装体形貌、粒径大小及波谱峰值特征。其次,通过紫外分光光度法研究了Res的药物缓释行为。最后,通过MTT法在人脐静脉内皮细胞(HUVECs)层面验证了Res/C60纳米组装体的安全性以及体外的抗炎性。研究发现,Res/C60纳米组装体呈短棒状,横截面平均直径为(379.74±33.47)nm。拉曼光谱和X射线衍射特征峰显示Res/C60纳米组装体被成功制备。Res/C60纳米组装体的聚氨酯(PU)涂层中Res药物在30 d内累积释放量可达60%左右。胆管支架涂层对HUVECs细胞毒性较低,且通过释放Res可显著抑制炎症因子的释放。The molecular self-assembly technology is used to assemble resveratrol(Res)and fullerene(C60)with into nanostructures,and polyurethane(PU)is used as the carrier for anti-inflammatory bile duct stents coating field.The Res/C60 nano-assembly has a short rod shape with an average cross-sectional diameter of 379.74±33.47 nm.The results of Raman spectroscopy and X-ray diffraction patterns prove that the Res/C60 nano-assembly is successfully prepared.The Res drug release amount of the PU coating loaded Res/C60 nano-assemblies showed the cumulative release amount was up to about 60%within 30 days.The cytotoxicity test and anti-inflammatory experiment show that the coating is less cytotoxic to HUVECs,and it can significantly inhibit the release of inflammatory factors by releasing Res.

关 键 词：白藜芦醇 富勒烯 自组装 抗炎 药物递送载体 

分 类 号：R318.08[医药卫生—生物医学工程]