宁麦9号/扬麦158重组自交系群体产量性状的遗传解析  

Genetic analysis for yield related traits of wheat(Triticum aestivum L.)based on a recombinant inbred line population from Ningmai 9 and Yangmai 158

作　　者：姜朋[1,2] 张旭[1,3] 吴磊 何漪[1] 张平平 马鸿翔[1] 孔令让[2] JIANG Peng;ZHANG Xu;WU Lei;HE Yi;ZHANG Ping-Ping;MA Hong-Xiang;KONG Ling-Rang(Provincial Key Laboratory for Agrobiology/Jiangsu Academy of Agricultural Sciences,Nanjing 210014,Jiangsu,China;State Key Laboratory of Crop Biology/Shandong Agricultural University,Tai’an 271018,Shandong,China;Co-Innovation Center for Modern Production Technology of Grain Crops Wheat Research Center/Yangzhou University,Yangzhou 225009,Jiangsu,China)

机构地区：[1]江苏省农业生物学重点实验室/江苏省农业科学院,江苏南京210014 [2]作物生物学国家重点实验室/山东农业大学,山东泰安271018 [3]江苏省粮食作物现代产业技术协同创新中心/扬州大学,江苏扬州225009

出　　处：《作物学报》2021年第5期869-881,共13页Acta Agronomica Sinica

基　　金：国家重点研发计划项目(2017YFD0100801);江苏省农业科技自主创新资金(CX(18)1001);江苏省重点研发计划项目(BE2019377);国家现代农业产业技术体系建设专项(CARS-3)资助。

摘　　要：宁麦9号与扬麦158是我国长江中下游麦区的主栽品种和骨干亲本,二者都为高产类型品种,但产量结构类型不同,研究二者产量及构成因素的遗传对产量遗传改良具重要意义。本研究以宁麦9号与扬麦158为亲本构建的包含282个家系的重组自交系群体为材料,利用Illumina 90K芯片分析遗传群体基因型,建立了高密度遗传图谱。连续3个生长季对产量及构成要素进行表型鉴定,结果显示,3年中宁麦9号穗粒数均高于扬麦158,千粒重低于扬麦158,穗数与产量在不同环境中表现不一致,在产量及其构成要素中,千粒重的遗传力最高。表型结合遗传作图对产量及构成因素进行分子定位,获得控制穗数、穗粒数、千粒重及产量的QTL分别为9、8、10与12个。为了将检测到的QTL有效地应用于标记辅助选择,将重复检测到的3个千粒重QTL相关标记转化成适用于高通量基因分型的KASP标记,并在育种料中进行了验证,3个QTL位点均具有较好的选择效果,且具有累加效应。上述研究结果可为长江中下游麦区小麦产量性状的分子育种提供理论依据和技术支撑。Ningmai 9 and Yangmai 158 are the main commercial wheat varieties,as well as the backbone parents in wheat breeding programs in the middle and lower reaches of the Yangtze River in China.Both of them have high yield potential,while they showed significant difference in yield components.To understand the genetic mechanism of their high yield potential,282 recombinant inbred lines(RILs)derived from the cross between Ningmai 9 and Yangmai 158 were genotyped with the Illumina iSelect 90 K wheat single nucleotide polymorphism(SNP)assay to construct a high-density genetic map.Yield(YD)and yield-related traits including spike number(SN),kernel number per spike(KN),and 1000-kernel weight(TKW)were evaluated for three consecutive growing seasons.The results indicated that the KN of Ningmai 9 was higher than that of Yangmai 158,while TKW was lower than that of Yangmai 158.In total,9,8,10,and 12 QTLs associated with SN,KN,TKW and YD were identified by QTL mapping,respectively.Among these traits,TKW possessed the highest heritability,and three QTL associated with TKW were identified repetitively.The markers related to such QTLs were then transferred to Kompetitive Allele Specific PCR(KASP)markers for high throughput selection in 139 wheat accessions.It was indicated that pyramiding 2 or 3 QTLs was more effective than single QTL for TKW improvement.The results in this study could provide more information for marker assisted selection in wheat yield breeding program.

关 键 词：小麦 宁麦9号 扬麦158 产量 KASP标记 

分 类 号：S512.1[农业科学—作物学]