献血者和患者中抗-“Mia”筛查与分析  

Prevalence of anti-“Mi” in blood donors and patients, Zhongshan city

作　　者：李乔 孙爱农 段生宝[2] 吴泳伦 廖艳婷 方育如 杨志钊[3] LI Qiao;SUN Ainong;DUAN Shengbao;WU Yonglun;LIAO Yanting;FANG Yuru;YANG Zhizhao(Zhongshan Blood Center,Zhongshan 528400,China;Suzhou Institute of Biomedical Engineering and Technology;Zhongshan People's Hospital.Corresponding author:SUN Among)

机构地区：[1]中山市中心血站,广东中山528400 [2]中国科学院苏州生物医学工程技术研究所 [3]中山市人民医院

出　　处：《中国输血杂志》2021年第2期114-117,共4页Chinese Journal of Blood Transfusion

基　　金：中山市卫生健康局医学科研项目(2019J382)。

摘　　要：目的了解本地区人群抗-"Mia"(抗-Mia类抗体)的发生频率和性质,以及不同试验条件对人源性抗-"Mia"活性的影响。方法在3 587名本地区无偿献血者和患者血液标本中,采用微孔板盐水介质凝集法和聚凝胺法、以O型Mia抗原阳性红细胞筛查人源性抗-"Mia",再用试管法和微柱凝胶卡法复核。结果本组抗-"Mia"频率1.06%(38/3 587),其中IgM类占60.5%(23/38)、IgM+IgG类占39.5%(15/38);本地献血者与患者分别为0.61%(13/2 135)、1.72%(25/1 452)(P<0.01);带抗-"Mia"的人群中,有免疫史者占65.8%(25/38)。经GP.Vw红细胞鉴定,抗-"Mur"占57.9%(22/38),抗-"Mia"占42.1%(16/38)。抗-"Mia"检测孵育时间以10 min为宜。结论本地区献血者和患者中抗-"Mia"频率较高,带抗-"Mia"者中以有免疫史者为主;抗-Mia类抗体多含有盐水活性,部分为IgM+IgG混合性质;若在不规则抗体筛检细胞谱中加上含有Mia阳性红细胞,将有助于提高输血免疫安全性。Objective To understand the frequency and significance of anti-"Mia"(anti-"Mia" mixtures of antibodies) in local population in Zhongshan, and the influence of different experimental conditions on the activity of human anti-"Mia". Methods The microplate-based agglutination assay and polybrene method were used to screen anti-"Mia" in 3 587 blood samples from voluntary blood donors and patients using O type red blood cells with positive Mia antigen, then.rechecked by tube method and microcolumn gel card method. Results The frequency of anti-"Mia" was 1.06%(38/3 587), among which 60.5%(23/38) were IgM and 39.5%(15/38) were mixture of IgM and IgG;0.61%(13/2 135) in local blood donors and 1.72%(25/1 452) in patients(P<0.01). 65.8%(25/38) of the population carrying anti-"Mia" had a history of immunity. 57.9%(22/38) were identified to be anti-"Mur" and 42.1%(16/38) anti-"Mia" using GP.Vw erythrocyte. The appropriate incubation time for anti-"Mia" test was 10 min. Conclusion The frequency of anti-"Mia" was relatively high among blood donors and patients in Zhongshan, and most of the anti-"Mia" carriers had a history of immunity. Most anti-Mia antibodies were active in saline, and some of them were mixture of IgM and IgG. It may be helpful to include Mia positive red blood cells in the irregular antibody screening cell panel to improve the safety of blood transfusion.

关 键 词：抗-Mia类抗体 Mia抗原 抗-“Mia”频率 盐水活性 免疫史 

分 类 号：R457.11[医药卫生—治疗学]