全血冷藏储存时间不同对制备去白悬浮红细胞质量的影响  

Effect of storage duration of whole blood on the preparation of leukocyte-depleted red blood cells

作　　者：周静[1] ZHOU Jing(Luoyang Central Blood Station,Luoyang 471000,China)

机构地区：[1]洛阳市中心血站,河南洛阳471000

出　　处：《中国输血杂志》2021年第2期132-134,共3页Chinese Journal of Blood Transfusion

摘　　要：目的探讨新鲜全血4℃储存时间不同后制备去白悬浮红细胞的质量差异。方法全血采集后4℃冷藏储存,分别于全血采集当日(d0制备组)和第二日(d2制备组)制备去白悬浮红细胞。检测制备过程中白细胞残留量、血液过滤时间、滤器损失血量和去白悬浮红细胞容量,检测全血冷藏储存不同时间Hct、MCV、RBC、Hb数值,2组去白悬浮红细胞的pH值、K+、Na+、游离血红蛋白及血浆溶血率。结果 d0制备组和d2制备组的滤白时间分别为(7.67±1.95)min和(11.55±2.43)min,2组去白悬浮红细胞的容量分别为(305.21±21.95)mL和(311.11±21.08)mL(P<0.05),滤器损失血量和去白悬浮红细胞的白细胞残留量无差异(P>0.05),全血冷藏储存不同时间Hct、RBC和Hb的数值变化均无差异(P>0.05),采血后即刻、d0制备前及d2制备前MCV值分别为:(89.37±2.57) fL、(89.68±2.74)fL、(90.30±3.25) fL(P<0.05),2组去白悬浮红细胞的K+、Na+、pH值以及游离血红蛋白的数据无差异(P>0.05),d0制备组血浆溶血率低于d2制备组(P<0.05)。结论随着全血冷藏时间的延长,全血中红细胞体积增大,造成去白悬浮红细胞的容量差异和血浆溶血率的增加。建议全血采集后冷藏储存10 h内完成去白悬浮红细胞的制备。Objective To discuss the differences in the quality of leukocyte-depleted red blood cells(LDRBCs) prepared from fresh whole blood stored at 4℃ with different storage duration. Methods The whole blood was collected and stored at 4℃. LDRBCs were prepared on the day of collection(d0 preparation group) and the next day(d2 preparation group). The leukocyte residues, blood filtering time, blood loss during filtration, LDRBCs volume, and the Hct,MCV, RBC and Hb in the packed whole blood with different storage duration, and the K+, Na+, pH, free hemoglobin concentration(fHb) and hemolysis rate of plasma between two groups were analyzed and compared. Results The filtering time of d0 preparation group and d2 preparation group was(7.67±1.95) min and(11.55±2.43) min, respectively. The volume of LDRBCs in the two groups was(305.21±21.95) mL and(311.11±21.08) mL, respectively(P<0.05). Concerning blood loss during filtration and WBC residues, there was no difference between two groups(P>0.05). No significant differences could be noticed in the value of Hct, RBC and Hb during the storage period(P>0.05), irrespective of MCV(fL), which changed significantly when sampled immediately after blood collection(89.37±2.57), prior to preparation at day 0(89.68±2.74) and prior to preparation at day 2(90.30±3.25)(P<0.05). No difference in K+, Na+, pH value and fHb was notable between the two groups(P>0.05), while hemolysis rate of plasma in day 0 preparation group was lower than that of day 2 preparation group(P<0.05). Conclusion The RBC deformability, increasing with the extension of whole blood cold storage time, results in the differences in the volume of LDRBCs and incidence of hemolysis. LDRBCs preparation within 10-hour cold storage following whole blood collection is recommended.

关 键 词：冷藏储存 去白悬浮红细胞 质量 

分 类 号：R457.1[医药卫生—治疗学] R331.141[医药卫生—临床医学]